Properties of multivesicular release from mouse rod photoreceptors support transmission of single-photon responses
| Autor: | Greg D. Field, Wallace B. Thoreson, Asia L Sladek, Cassandra L. Hays |
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| Rok vydání: | 2021 |
| Předmět: |
Male
Mouse genetic structures QH301-705.5 Science scotopic vision Ribbon synapse Stimulus (physiology) General Biochemistry Genetics and Molecular Biology Exocytosis Membrane Potentials Mice rod photoreceptor cell Retinal Rod Photoreceptor Cells medicine Animals Scotopic vision Biology (General) Membrane potential Photons Retina General Immunology and Microbiology Chemistry General Neuroscience Vesicle General Medicine medicine.anatomical_structure synaptic vesicle release Darkness Biophysics Medicine Female Synaptic Vesicles sense organs Research Article Neuroscience |
| Zdroj: | eLife eLife, Vol 10 (2021) |
| ISSN: | 2050-084X |
| DOI: | 10.7554/elife.67446 |
| Popis: | Vision under starlight requires rod photoreceptors to transduce and transmit single-photon responses to the visual system. Small single-photon voltage changes must therefore cause detectable reductions in glutamate release. We found that rods achieve this by employing mechanisms that enhance release regularity and its sensitivity to small voltage changes. At the resting membrane potential in darkness, mouse rods exhibit coordinated and regularly timed multivesicular release events, each consisting of ~17 vesicles and occurring two to three times more regularly than predicted by Poisson statistics. Hyperpolarizing rods to mimic the voltage change produced by a single photon abruptly reduced the probability of multivesicular release nearly to zero with a rebound increase at stimulus offset. Simulations of these release dynamics indicate that this regularly timed, multivesicular release promotes transmission of single-photon responses to post-synaptic rod-bipolar cells. Furthermore, the mechanism is efficient, requiring lower overall release rates than uniquantal release governed by Poisson statistics. |
| Databáze: | OpenAIRE |
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