Injury-induced mechanical hypersensitivity requires C-low threshold mechanoreceptors
| Autor: | Yabo Guan, Xingmei Wang, Robert H. Edwards, Srinivasa N. Raja, Allan I. Basbaum, C. Jeffery Woodbury, Rebecca P. Seal |
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| Rok vydání: | 2009 |
| Předmět: |
Amino Acid Transport Systems
Acidic Population Pain Poison control Somatosensory system Article Mice Dorsal root ganglion Ganglia Spinal Hypersensitivity medicine Animals education Mice Knockout education.field_of_study Multidisciplinary Behavior Animal business.industry Glutamate receptor Nerve injury Spinal cord Sensory neuron Mice Inbred C57BL medicine.anatomical_structure Gene Expression Regulation Wounds and Injuries Female medicine.symptom business Mechanoreceptors Neuroscience |
| Zdroj: | Nature |
| ISSN: | 1476-4687 0028-0836 |
| Popis: | Mechanical pain contributes to the morbidity associated with inflammation and trauma, but primary sensory neurons that convey the sensation of acute and persistent mechanical pain have not been identified. Dorsal root ganglion (DRG) neurons transmit sensory information to the spinal cord using the excitatory transmitter glutamate, a process that depends on glutamate transport into synaptic vesicles for regulated exocytotic release. Here we report that a small subset of cells in the DRG expresses the low abundance vesicular glutamate transporter VGLUT3 (also known as SLC17A8). In the dorsal horn of the spinal cord, these afferents project to lamina I and the innermost layer of lamina II, which has previously been implicated in persistent pain caused by injury. Because the different VGLUT isoforms generally have a non-redundant pattern of expression, we used Vglut3 knockout mice to assess the role of VGLUT3(+) primary afferents in the behavioural response to somatosensory input. The loss of VGLUT3 specifically impairs mechanical pain sensation, and in particular the mechanical hypersensitivity to normally innocuous stimuli that accompanies inflammation, nerve injury and trauma. Direct recording from VGLUT3(+) neurons in the DRG further identifies them as a poorly understood population of unmyelinated, low threshold mechanoreceptors (C-LTMRs). The analysis of Vglut3(-/-) mice now indicates a critical role for C-LTMRs in the mechanical hypersensitivity caused by injury. |
| Databáze: | OpenAIRE |
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