An altered tocopherol composition in chloroplasts reduces plant resistance to Botrytis cinerea
| Autor: | Michael R. F. Lee, Anushen Sivakumaran, Sergi Munné-Bosch, John K. S. Tweed, Luis A. J. Mur, Jana Cela |
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| Rok vydání: | 2018 |
| Předmět: |
0106 biological sciences
0301 basic medicine Chloroplasts Physiology Mutant Arabidopsis Hormonal balance Tocopherols Plant Science 01 natural sciences Lipid peroxidation 03 medical and health sciences chemistry.chemical_compound Biotic stress Genetics Pseudomonas syringae Disease Resistance Botrytis cinerea Jasmonic acid biology Chemistry fungi Wild type food and beverages biology.organism_classification Lipid profile 030104 developmental biology Biochemistry Cytokinin lipids (amino acids peptides and proteins) Botrytis 010606 plant biology & botany |
| Zdroj: | Cela, J, Tweed, J K S, Sivakumaran, A, Lee, M R F, Mur, L A J & Munné-Bosch, S 2018, ' An altered tocopherol composition in chloroplasts reduces plant resistance to Botrytis cinerea ', Plant Physiology and Biochemistry, vol. 127, pp. 200-210 . https://doi.org/10.1016/j.plaphy.2018.03.033 |
| DOI: | 10.1016/j.plaphy.2018.03.033 |
| Popis: | Tocopherols are lipid-soluble antioxidants that contribute to plant resistance to abiotic stresses. However, it is still unknown to what extent alterations in tocopherol composition can affect the plant response to biotic stresses. The response to bacterial and fungal attack of the vte1 mutant of Arabidopsis thaliana, which lacks both α- and γ-tocopherol, was compared to that of the vte4 mutant (which lacks α- but accumulates γ-tocopherol) and the wild type (with accumulates α-tocopherol in leaves). Both mutants exhibited similar kinetics of cell death and resistance in response to Pseudomonas syringae. In contrast, both mutants exhibited delayed resistance when infected with Botrytis cinerea. Lipid and hormonal profiling was employed with the aim of assessing the underlying cause of this differential phenotype. Although an altered tocopherol composition in both mutants strongly influenced fatty acid composition, and strongly altered jasmonic acid and cytokinin contents upon infection with B. cinerea, differences between genotypes in these phytohormones were observed during late stages of infection only. By contrast, genotype-related effects on lipid peroxidation, as indicated by malondialdehyde accumulation, were observed early upon infection with B. cinerea. We conclude that an altered tocopherol composition in chloroplasts may negatively influence the plant response to biotic stress in Arabidopsis thaliana through changes in the membrane fatty acid composition, enhanced lipid peroxidation and delayed defence activation when challenged with B. cinerea. |
| Databáze: | OpenAIRE |
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