Placental gene transfer: transgene screening in mice for trophic effects on the placenta
| Autor: | Elliott D. Kozin, Foong-Yen Lim, Timothy M. Crombleholme, Philip W. Zoltick, Paola Midrio, Anna B. Katz, Sundeep G. Keswani, Meenhard Herlyn, Mounira Habli |
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| Rok vydání: | 2009 |
| Předmět: |
intrauterine growth restriction
Placenta Transgene medicine.medical_treatment Basic fibroblast growth factor Intrauterine growth restriction Biology Article Andrology Mice chemistry.chemical_compound Pregnancy growth factors medicine Animals reproductive and urinary physiology Mice Inbred BALB C Fetus business.industry Growth factor Gene Transfer Techniques Obstetrics and Gynecology medicine.disease gene therapy Embryonic stem cell Vascular endothelial growth factor medicine.anatomical_structure chemistry embryonic structures Immunology placenta Intercellular Signaling Peptides and Proteins Female Hepatocyte growth factor business medicine.drug |
| Zdroj: | American Journal of Obstetrics and Gynecology. 201:499.e1-499.e8 |
| ISSN: | 0002-9378 |
| DOI: | 10.1016/j.ajog.2009.06.029 |
| Popis: | Objective We hypothesized that gene transfer of select growth factors to the placenta may enhance placental and fetal growth. Thus, we examined the effect of 8 growth factor transgenes on murine placenta. Study Design Adenoviral-mediated site-specific intraplacental gene transfer of 8 different growth factor transgenes at embryonic day (e) 14 was performed. Transgenes included angiopoietin-1, angiopoietin-2 (Ang-2), basic fibroblast growth factor, hepatocyte growth factor, insulin-like growth factor-1 (IGF-1), placenta growth hormone, platelet-derived growth factor-B (PDGF-B), and vascular endothelial growth factor 121 . Fetuses and placentas were harvested at e17 and assessed for survival, gene transfer efficiency, placenta area, and fetal and placental weights. Results Efficient gene transfer to the placenta was detected with minimal dissemination to the fetus. Overexpression of IGF-1, PDGF-B, and Ang-2 resulted in an increase in placenta cross-sectional area. Only Ang-2 gene transfer resulted in increased fetal weight, and only Ang-2 and basic fibroblast growth factor resulted in a change in placental weight. Conclusion Site-specific placental gene transfer results in efficient gene transfer with minimal dissemination to the fetus. Adenoviral-mediated IGF-1, adenoviral-mediated PDGF-B, and adenoviral-mediated Ang-2 significantly increase placenta growth. |
| Databáze: | OpenAIRE |
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