Transcriptomic analysis of inbreeding depression in cold-sensitive Drosophila melanogaster shows upregulation of the immune response

Autor: Cornelis J. Vermeulen, Volker Loeschcke, K. Kirilova Gagalova, Peter Sørensen
Jazyk: angličtina
Rok vydání: 2013
Předmět:
Male
cold resistance
serine proteases
INTEGRATIVE ANALYSIS
ENVIRONMENTAL-STRESS
CASCADES
Transcriptome
Stress
Physiological
PCR DATA
Gene expression
medicine
Inbreeding depression
Animals
Cluster Analysis
Inbreeding
alpha-mannosidase
Gene–environment interaction
GENOME-WIDE ANALYSIS
Gene
innate immunity
Ecology
Evolution
Behavior and Systematics
Oligonucleotide Array Sequence Analysis
GENE-EXPRESSION
Genetics
biology
Reverse Transcriptase Polymerase Chain Reaction
BIOINFORMATICS
serpins
thermal resistance
Computational Biology
WILD
biology.organism_classification
Immunity
Innate
MICROARRAYS
Cold Temperature
Drosophila melanogaster
Gene Expression Regulation
gene expression
Cold sensitivity
POPULATIONS
genotype-by-environment interaction
medicine.symptom
conditional lethality
inbreeding depression
Zdroj: Vermeulen, C J, Sørensen, P, Kirilova Gagalova, K & Loeschcke, V 2013, ' Transcriptomic analysis of inbreeding depression in cold-sensitive Drosophila melanogaster shows upregulation of the immune response ', Journal of Evolutionary Biology, vol. 26, no. 9, pp. 1890-1902 . https://doi.org/10.1111/jeb.12183
Journal of Evolutionary Biology, 26(9), 1890-1902. Wiley
ISSN: 1010-061X
Popis: In sexually reproducing species, increased homozygosity often causes a decline in fitness, called inbreeding depression. Recently, researchers started describing the functional genomic changes that occur during inbreeding, both in benign conditions and under environmental stress. To further this aim, we have performed a genome-wide gene expression study of inbreeding depression, manifesting as cold sensitivity and conditional lethality. Our focus was to describe general patterns of gene expression during inbreeding depression and to identify specific processes affected in our line. There was a clear difference in gene expression between the stressful restrictive environment and the benign permissive environment in both the affected inbred line and the inbred control line. We noted a strong inbreeding-by-environment interaction, whereby virtually all transcriptional differences between lines were found in the restrictive environment. Functional annotation showed enrichment of transcripts coding for serine proteases and their inhibitors (serpins and BPTI/Kunitz family), which indicates activation of the innate immune response. These genes have previously been shown to respond transcriptionally to cold stress, suggesting the conditional lethal effect is associated with an exaggerated cold stress response. The set of differentially expressed genes significantly overlapped with those found in three other studies of inbreeding depression, demonstrating that it is possible to detect a common signature across different genetic backgrounds.
Databáze: OpenAIRE
Externí odkaz: