Comparison of aqueous soluble proteins profile of Mycobacterium tuberculosis H37Rv and H37Ra and a Malaysian clinical isolate
| Autor: | Gam Lay-Harn, Ang Kai-Cheen |
|---|---|
| Rok vydání: | 2018 |
| Předmět: |
0301 basic medicine
030106 microbiology Biomedical Engineering Virulence Bioengineering Proteomics Applied Microbiology and Biotechnology Microbiology Mycobacterium tuberculosis 03 medical and health sciences Bacterial Proteins Tandem Mass Spectrometry Drug Discovery Tuberculosis Electrophoresis Gel Two-Dimensional Gel electrophoresis Two-dimensional gel electrophoresis biology Process Chemistry and Technology Malaysia Water Lipid metabolism General Medicine Metabolism biology.organism_classification Solubility Molecular Medicine Biomarkers Biotechnology Mycobacterium |
| Zdroj: | Biotechnology and applied biochemistry. 65(6) |
| ISSN: | 1470-8744 |
| Popis: | Differentially expressed aqueous soluble proteins between Mycobacterium tuberculosis H37Ra and H37Rv were identified. The protein extracts were separated by two-dimensional gel electrophoresis followed by tandem mass spectrometric analysis. Twelve proteins were detected to be differentially expressed significantly between virulent strain H37Rv and attenuated strain H37Ra. The differentially expression of these proteins was validated by a recently isolated clinical virulent strains of M. tuberculosis, TB138. Out of the 12 proteins identified, which consisted of ten upregulated and two downregulated proteins, nine were belonged to intermediate metabolism and respiration protein group, two were in lipid metabolism, and one protein was involved in information pathways and virulence. Among these proteins, two of the upregulated proteins, namely, mmsA and pntAa, showed a consistent expression pattern in both virulent mycobacterium strains. These proteins can serve as potential biomarkers for the intervention treatment of TB. |
| Databáze: | OpenAIRE |
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