Mitotic centromere-associated kinase (MCAK/Kif2C) regulates cellular senescence in human primary cells through a p53-dependent pathway
| Autor: | Mi-Ri Gwon, Jae-Ryong Kim, Jung Hee Cho |
|---|---|
| Rok vydání: | 2012 |
| Předmět: |
p53
Senescence Chromosome movement Blotting Western Cell Biophysics Kinesins Mitosis Biology Biochemistry Cell Line Structural Biology Microtubule Genetics medicine Humans Molecular Biology Cells Cultured Cellular Senescence Gene knockdown Human primary cells Reverse Transcriptase Polymerase Chain Reaction Kinase Cell Biology Mitotic centromere-associated kinase (MCAK/Kif2C) Cell biology medicine.anatomical_structure Cell culture Cancer research Tumor Suppressor Protein p53 Signal Transduction |
| Zdroj: | FEBS Letters. 586:4148-4156 |
| ISSN: | 0014-5793 |
| Popis: | Mitotic centromere-associated kinase (MCAK/Kif2C) plays a critical role in chromosome movement and segregation with ATP-dependent microtubule depolymerase activity. However, its role in cellular senescence remains unclear. MCAK/Kif2C expression decreased in human primary cells under replicative and premature senescence. MCAK/Kif2C down-regulation in young cells induced premature senescence. MCAK/Kif2C overexpression in old cells partially reversed cell senescence. Senescence phenotypes by MCAK/Kif2C knockdown were observed in p16-knockdown cells, but not in p53-knockdown cells. These results suggest that MCAK/Kif2C plays an important role in the regulation of cellular senescence through a p53-dependent pathway and might contribute to tissue/organism aging and protection of cellular transformation. |
| Databáze: | OpenAIRE |
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