New chiral reverse phase HPLC method for enantioselective analysis of ketorolac using chiral AGP column
| Autor: | S. Pasha, K Chiranjeevi Venkatesh, Sunil Kumar Dubey, Jangala Hemanth, R. N. Saha |
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| Rok vydání: | 2012 |
| Předmět: |
R-Ketorolac
Chiral AGP Pharmaceutical Science Pharmacy Article Analytical Chemistry Sodium phosphate buffer Phase (matter) Drug Discovery Electrochemistry medicine Spectroscopy Medicine(all) Chromatography Biochemistry Genetics and Molecular Biology(all) Chemistry lcsh:RM1-950 S-Ketorolac Enantioselective synthesis Linearity Reversed-phase chromatography Ketorolac lcsh:Therapeutics. Pharmacology RP-HPLC Phase composition Enantiomer medicine.drug |
| Zdroj: | Journal of Pharmaceutical Analysis Journal of Pharmaceutical Analysis, Vol 2, Iss 6, Pp 462-465 (2012) |
| ISSN: | 2095-1779 |
| DOI: | 10.1016/j.jpha.2012.07.006 |
| Popis: | A simple, specific, precise, sensitive and rapid reverse phase-HPLC method was developed for determination of ketorolac enantiomers, a potent nonnarcotic analgesic in pharmaceutical formulations. The method was developed on a chiral AGP column. Mobile phase was 0.1 M sodium phosphate buffer (pH 4.5): Isopropanol (98:2, v/v), at a flow rate of 1 mL/min with run time of 15 min. Ultraviolet detection was made at 322 nm. The linearity range was 0.02â10 μg/mL for each of the enantiomers. The mobile phase composition was systematically studied to find the optimum chromatographic conditions. Validation of the method under the conditions selected showed that it was selective and precise and that the detector response was linear function of ketorolac. Keywords: R-Ketorolac, S-Ketorolac, Chiral AGP, RP-HPLC |
| Databáze: | OpenAIRE |
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