Spheroid model study comparing the biocompatibility of Biodentine and MTA
| Autor: | M Pérard, Sylvie Tricot-Doleux, Justine Le Clerc, Fabienne Pérez, Fleur Meary, Pascal Pellen-Mussi |
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| Rok vydání: | 2012 |
| Předmět: |
Mineral trioxide aggregate
Pathology medicine.medical_specialty Biocompatibility Cell Survival Cellular differentiation Biomedical Engineering Biophysics Bioengineering Biocompatible Materials Cell Line Biomaterials Andrology Root Canal Filling Materials stomatognathic system Spheroids Cellular medicine Humans Aluminum Compounds Dental Pulp biology Odontoblasts Silicates Spheroid Acid phosphatase Oxides Calcium Compounds RUNX2 Drug Combinations Cell culture biology.protein Pulp (tooth) Pulp Capping and Pulpectomy Agents |
| Zdroj: | Journal of materials science. Materials in medicine. 24(6) |
| ISSN: | 1573-4838 |
| Popis: | The primary objective of this study was to assess the biological effects of a new dentine substitute based on Ca₃SiO₅ (Biodentine™) for use in pulp-capping treatment, on pseudo-odontoblastic (MDPC-23) and pulp (Od-21) cells. The secondary objective was to evaluate the effects of Biodentine and mineral trioxide aggregate (MTA) on gene expression in cultured spheroids. We used the acid phosphatase assay to compare the biocompatibility of Biodentine and MTA. Cell differentiation was investigated by RT-qPCR. We investigated the expression of genes involved in odontogenic differentiation (Runx2), matrix secretion (Col1a1, Spp1) and mineralisation (Alp). ANOVA and PLSD tests were used for data analysis. MDPC-23 cells cultured in the presence of MTA had higher levels of viability than those cultured in the presence of Biodentine and control cells on day 7 (P = 0.0065 and P = 0.0126, respectively). For Od-21 cells, proliferation rates on day 7 were significantly lower in the presence of Biodentine or MTA than for control (P < 0.0001). Col1a1 expression levels were slightly lower in cells cultured in the presence of MTA than in those cultured in the presence of Biodentine and in control cells. Biodentine and MTA may modify the proliferation of pulp cell lines. Their effects may fluctuate over time, depending on the cell line considered. The observed similarity between Biodentine and MTA validates the indication for direct pulp-capping claimed by the manufacturers. |
| Databáze: | OpenAIRE |
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