Early apoptosis of macrophages modulated by injection of Yersinia pestis YopK promotes progression of primary pneumonic plague
| Autor: | Miqdad O. Dhariwala, Deborah M. Anderson, Jennifer M. Hughes Hanks, Charles R. Brown, Kristen N. Peters |
|---|---|
| Jazyk: | angličtina |
| Rok vydání: | 2013 |
| Předmět: |
Male
Yersinia pestis Apoptosis Type three secretion system Mice Gram Negative Biology (General) Promoter Regions Genetic Bacterial Secretion Systems Caspase 8 Mice Inbred BALB C 0303 health sciences biology Caspase 3 Effector Pyroptosis Innate Immunity Bacterial Pathogens 3. Good health Host-Pathogen Interaction Female medicine.symptom Research Article Pneumonic plague QH301-705.5 Immunology Inflammation Microbiology 03 medical and health sciences Immune system Bacterial Proteins Virology Genetics medicine Animals Biology Microbial Pathogens Molecular Biology 030304 developmental biology Plague 030306 microbiology Macrophages Immunity Immune Defense Dendritic Cells RC581-607 biology.organism_classification medicine.disease Enzyme Activation Mice Inbred C57BL Emerging Infectious Diseases Parasitology Immunologic diseases. Allergy Apoptosis Regulatory Proteins |
| Zdroj: | PLoS Pathogens, Vol 9, Iss 4, p e1003324 (2013) PLoS Pathogens |
| ISSN: | 1553-7374 1553-7366 |
| Popis: | Yersinia pestis causes pneumonic plague, a disease characterized by inflammation, necrosis and rapid bacterial growth which together cause acute lung congestion and lethality. The bacterial type III secretion system (T3SS) injects 7 effector proteins into host cells and their combined activities are necessary to establish infection. Y. pestis infection of the lungs proceeds as a biphasic inflammatory response believed to be regulated through the control of apoptosis and pyroptosis by a single, well-conserved T3SS effector protein YopJ. Recently, YopJ-mediated pyroptosis, which proceeds via the NLRP3-inflammasome, was shown to be regulated by a second T3SS effector protein YopK in the related strain Y. pseudotuberculosis. In this work, we show that for Y. pestis, YopK appears to regulate YopJ-mediated apoptosis, rather than pyroptosis, of macrophages. Inhibition of caspase-8 blocked YopK-dependent apoptosis, suggesting the involvement of the extrinsic pathway, and appeared cell-type specific. However, in contrast to yopJ, deletion of yopK caused a large decrease in virulence in a mouse pneumonic plague model. YopK-dependent modulation of macrophage apoptosis was observed at 6 and 24 hours post-infection (HPI). When YopK was absent, decreased populations of macrophages and dendritic cells were seen in the lungs at 24 HPI and correlated with resolution rather than progression of inflammation. Together the data suggest that Y. pestis YopK may coordinate the inflammatory response during pneumonic plague through the regulation of apoptosis of immune cells. Author Summary In this work, we studied the mechanism whereby bacteria manipulate innate immune responses by controlling host cell death. Yersinia pestis, the causative agent of plague, requires effector Yops of the Type III Secretion System (T3SS) to evade the innate immune system during infection. We show that Yersinia induces apoptosis of macrophages through two distinct mechanisms, each through the activity of the well-characterized T3SS effector YopJ, yet regulated in an opposing manner through the activity of a second effector protein YopK. In a murine pneumonic plague model, we found evidence that YopK regulates apoptosis of macrophages during the early stage of infection, leading to uncontrolled inflammation and disease. In contrast, the absence of YopK-regulated apoptosis allowed recruitment of lymphocytes and CCR2+ immune cells which led to bacterial clearance and resolution of inflammation. Together the data suggest that Yersinia YopK modulates apoptosis of immune cells to control the inflammatory response during plague. |
| Databáze: | OpenAIRE |
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