Direct immobilization of manganese chelates on silica nanospheres for MRI applications
| Autor: | Judith Mihály, Katalin Jemnitz, Krisztián Szigeti, Zoltán May, Marcell Pálmai, Adrienn Pethő, Domokos Máthé, Lívia Naszályi Nagy, Zoltán Varga, András Wacha, Szilvia Klébert, Zsuzsanna Veres, Ildiko Horvath |
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| Rok vydání: | 2017 |
| Předmět: |
Male
Cell Survival Surface Properties Contrast Media 02 engineering and technology 010402 general chemistry 01 natural sciences Cell Line Biomaterials chemistry.chemical_compound Colloid and Surface Chemistry Nuclear magnetic resonance Pulmonary surfactant In vivo Specific surface area medicine Animals Humans Chelation Amines Particle Size Rats Wistar Chelating Agents Manganese Aqueous solution medicine.diagnostic_test Chemistry Magnetic resonance imaging Microporous material Pentetic Acid 021001 nanoscience & nanotechnology Silicon Dioxide Magnetic Resonance Imaging 0104 chemical sciences Surfaces Coatings and Films Electronic Optical and Magnetic Materials Mice Inbred C57BL Silanol Liver Hepatocytes 0210 nano-technology Porosity Nanospheres Nuclear chemistry |
| Zdroj: | Journal of colloid and interface science. 498 |
| ISSN: | 1095-7103 |
| Popis: | The development of tissue specific magnetic resonance imaging (MRI) contrast agents (CAs) is very desirable to achieve high contrast ratio combined with excellent anatomical details. To this end, we introduce a highly effective manganese(II) containing silica material, with the aim to shorten the longitudinal (T1) relaxation time. The microporous silica nanospheres (MSNSs) with enlarged porosity and specific surface area were prepared by a surfactant assisted aqueous method. Subsequently, the surface silanol groups were amino-functionalized, reacted with diethylenetriaminepentaacetic (DTPA) dianhydride and finally deposited with Mn2+. After comprehensive characterization, the MRI properties of functionalized MSNSs were investigated. The resulting nanospheres demonstrated substantial contrast enhancement during the in vitro MRI investigations, which was also evidenced by significant contrast enhancement on T1-weighted MR images in vivo. Moreover, in vitro cytotoxicity assay of functionalized MSNSs on hepatocyte mono- and hepatocyte-Kuppfer cell co-cultures showed no significant decrease in cell viability. Our findings confirmed our hypothesis, that Mn2+-chelating MSNSs are appropriate candidates for liver-specific T1-weighted MRI CAs with high relaxivities (r1=7.18mM-1s-1). |
| Databáze: | OpenAIRE |
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