p53 expression in repair/reactive renal tubular cells: A potential pitfall leading to a false‐positive diagnosis of urine cytology
| Autor: | Toru Matsunaga, Eiichiro Hirakawa, Shingo Kamoshida, Emi Ibuki, Kaori Enomoto, Akihiro Nakamura, Tadashi Sofue, Reiji Haba, Hiroyuki Ohsaki |
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| Rok vydání: | 2021 |
| Předmět: |
Male
p53 Cancer Research Pathology medicine.medical_specialty Cytodiagnosis Immunocytochemistry glomerular disease Urine immunocytochemistry urine cytology Cytology Biomarkers Tumor medicine Humans False Positive Reactions Radiology Nuclear Medicine and imaging RC254-282 Research Articles Homogentisate 1 2-dioxygenase Urine cytology Carcinoma Transitional Cell medicine.diagnostic_test biology business.industry Neoplasms. Tumors. Oncology. Including cancer and carcinogens Clinical Cancer Research renal tubular cell Middle Aged Immunohistochemistry Oncology biology.protein Female Kidney Diseases Renal biopsy Tumor Suppressor Protein p53 Antibody business Immunostaining Research Article |
| Zdroj: | Cancer Medicine Cancer Medicine, Vol 10, Iss 24, Pp 8846-8853 (2021) |
| ISSN: | 2045-7634 |
| DOI: | 10.1002/cam4.4389 |
| Popis: | Background p53 immunostaining is routinely used as a surrogate marker for TP53 mutational status. In urine cytology, p53 immunocytochemistry is reportedly useful in detecting urothelial carcinoma cells as well as in improving the detection sensitivity and specificity. However, to the best of our knowledge, p53 expression in repair/reactive renal tubular cells (RRTCs) from urine cytologic specimens has not been assessed to date. Methods We evaluated the immunoexpression of p53 and homogentisate 1,2‐dioxygenase (HGD) antibody, a renal tubular cells marker, in RRTCs using voided urine and renal biopsy samples from 80 patients who were histologically diagnosed with glomerular disease. Results Repair/reactive renal tubular cells were detected in 68 (68/80, 85%) samples at a mean count of 141.1 cells per sample (range, 5–4220). Immunocytochemical analysis found p53‐positive RRTCs in all the samples (68/68, 100%) with an average p53 positivity rate of RRTCs per sample at 47.7% (range, 3.8%–96.5%). Of the 68 p53‐positive RRTC samples, 38 (55.9%) included cells that were HGD positive for cytoplasm. Similarly, renal biopsy analysis revealed p53‐positive RRTCs in all the specimens (68/68, 100%). All 68 (100%) cases showed RRTCs that were positive for both p53 and HGD. Conclusion To avoid false positives of p53 immunocytochemistry, cytologists must consider the fact that RRTCs from patients with glomerular disease are positive for p53. Although the existence and cytological features of repair/reactive renal tubular cells in urine are not yet widely well known, these cells cytomorphologically mimic cancer cells. This study found that repair/reactive renal tubular cells are positive for p53 immunocytochemistry, which is crucial information for helping to avoid false positives in urine cytology. |
| Databáze: | OpenAIRE |
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