Comparative transcriptome profiling identifies maize line specificity of fungal effectors in the maize‐ Ustilago maydis interaction
| Autor: | Selma Schurack, Marco Thines, Deepak K. Gupta, Jasper R L Depotter, Gunther Doehlemann |
|---|---|
| Rok vydání: | 2021 |
| Předmět: |
0106 biological sciences
Ustilago Virulence Plant Science Plant disease resistance Genes Plant Zea mays 01 natural sciences Transcriptome 03 medical and health sciences Pathosystem CRISPR-Associated Protein 9 Genetics Gene Disease Resistance Plant Diseases 030304 developmental biology Gene Editing 2. Zero hunger 0303 health sciences biology Effector Basidiomycota Gene Expression Profiling Cell Biology biology.organism_classification Host-Pathogen Interactions Smut CRISPR-Cas Systems 010606 plant biology & botany |
| Zdroj: | The Plant Journal |
| ISSN: | 0960-7412 |
| DOI: | 10.1111/tpj.15195 |
| Popis: | The biotrophic pathogen Ustilago maydis causes smut disease on maize (Zea mays) and induces the formation of tumours on all aerial parts of the plant. Unlike in other biotrophic interactions, no gene-for-gene interactions have been identified in the maize-U. maydis pathosystem. Thus, maize resistance to U. maydis is considered a polygenic, quantitative trait. Here, we study the molecular mechanisms of quantitative disease resistance (QDR) in maize, and how U. maydis interferes with its components. Based on quantitative scoring of disease symptoms in 26 maize lines, we performed an RNA sequencing (RNA-Seq) analysis of six U. maydis-infected maize lines of highly distinct resistance levels. The different maize lines showed specific responses of diverse cellular processes to U. maydis infection. For U. maydis, our analysis identified 406 genes being differentially expressed between maize lines, of which 102 encode predicted effector proteins. Based on this analysis, we generated U. maydis CRISPR/Cas9 knock-out mutants for selected candidate effector sets. After infections of different maize lines with the fungal mutants, RNA-Seq analysis identified effectors with quantitative, maize line-specific virulence functions, and revealed auxin-related processes as a possible target for one of them. Thus, we show that both transcriptional activity and virulence function of fungal effector genes are modified according to the infected maize line, providing insights into the molecular mechanisms underlying QDR in the maize-U. maydis interaction. |
| Databáze: | OpenAIRE |
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