Neuroprotective effects of Ganoderma lucidum polysaccharides against traumatic spinal cord injury in rats
| Autor: | Ramazan Kahveci, Osman Malik Atanur, Bora Gürer, Aysun Gökçe, Nurkan Aksoy, Berker Cemil, Bulent Erdogan, Emre Cemal Gokce, Mustafa F. Sargon, Ozan Kahveci |
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| Přispěvatelé: | Zonguldak Bülent Ecevit Üniversitesi, Kırıkkale Üniversitesi |
| Rok vydání: | 2015 |
| Předmět: |
Male
Pathology medicine.medical_specialty Reishi Necrosis medicine.medical_treatment Ganoderma lucidum Spinal cord injury Methylprednisolone Trauma Neuroprotection Nitric oxide chemistry.chemical_compound Polysaccharides medicine Animals Rats Wistar Spinal Cord Injuries General Environmental Science Traditional medicine business.industry Laminectomy medicine.disease Malondialdehyde Spinal cord Rats Disease Models Animal Neuroprotective Agents medicine.anatomical_structure Spinal Cord chemistry General Earth and Planetary Sciences medicine.symptom business medicine.drug |
| Zdroj: | Injury. 46:2146-2155 |
| ISSN: | 0020-1383 |
| DOI: | 10.1016/j.injury.2015.08.017 |
| Popis: | Gurer, Bora/0000-0003-1500-6184; Sargon, Mustafa Fevzi/0000-0001-6360-6008 WOS: 000363901600010 PubMed: 26298021 Introduction: Ganoderma lucidum (G. lucidum) is a mushroom belonging to the polyporaceae family of Basidiomycota and has widely been used as a traditional medicine for thousands of years. G. lucidum has never been studied in traumatic spinal cord injury. The aim of this study is to investigate whether G. lucidum polysaccharides (GLPS) can protect the spinal cord after experimental spinal cord injury. Materials and methods: Rats were randomized into five groups of eight animals each: control, sham, trauma, GLPS, and methylprednisolone. In the control group, no surgical intervention was performed. In the sham group, only a laminectomy was performed. In all the other groups, the spinal cord trauma model was created by the occlusion of the spinal cord with an aneurysm clip. In the spinal cord tissue, caspase-3 activity, tumour necrosis factor-alpha levels, myeloperoxidase activity, malondialdehyde levels, nitric oxide levels, and superoxide dismutase levels were analysed. Histopathological and ultrastructural evaluations were also performed. Neurological evaluation was performed using the Basso, Beattie, and Bresnahan locomotor scale and the inclined-plane test. Results: After traumatic spinal cord injury, increases in caspase-3 activity, tumour necrosis factor-alpha levels, myeloperoxidase activity, malondialdehyde levels, and nitric oxide levels were detected. After the administration of GLPS, decreases were observed in tissue caspase-3 activity, tumour necrosis factor-alpha levels, myeloperoxidase activity, malondialdehyde levels, and nitric oxide levels. Furthermore, GLPS treatment showed improved results in histopathological scores, ultrastructural scores, and functional tests. Conclusions: Biochemical, histopathological, and ultrastructural analyses and functional tests reveal that GLPS exhibits meaningful neuroprotective effects against spinal cord injury. (C) 2015 Elsevier Ltd. All rights reserved. |
| Databáze: | OpenAIRE |
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