Piglet production by non‐surgical transfer of vitrified embryos, transported to commercial swine farms and warmed on site
| Autor: | Shigeyuki Tajima, Hiroyasu Misawa, Rie Takishita, Kazuhiro Kikuchi, Yuichiro Wakiya, Yuri Hirayama, Sawako Motoyama, Kenzo Uchikura |
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| Rok vydání: | 2020 |
| Předmět: |
animal diseases
medicine.medical_treatment Biology non‐surgical porcine embryo transfer vitrified and warmed embryos 03 medical and health sciences Animal science one‐step warming and dilution Proximal site medicine 030304 developmental biology Estrous cycle 0303 health sciences Artificial insemination artificial insemination 0402 animal and dairy science Embryo Original Articles 04 agricultural and veterinary sciences General Medicine 040201 dairy & animal science Embryo transfer Surgical transfer embryonic structures Original Article General Agricultural and Biological Sciences commercial farm |
| Zdroj: | Animal Science Journal = Nihon Chikusan Gakkaiho |
| ISSN: | 1740-0929 1344-3941 |
| Popis: | We investigated the feasibility of piglet production by non‐surgical embryo transfer (Ns‐ET) of vitrified porcine blastocysts and expanded blastocysts transported to commercial farms and warmed on site (V/T/W embryos). Ns‐ET was performed by depositing 11–20 vitrified and warmed embryos at a proximal site within the uterus via a catheter. In Experiment 1, the effect of donor–recipient estrous cycle asynchrony on the efficiency of Ns‐ET of vitrified and ordinary warmed embryos was investigated at the experimental facility. With a 1‐day delay recipients relative to that of donor, the farrowing rate was 50.0% and the survival rate to term was 21.1%. In Experiment 2, Ns‐ET using recipients with a 1‐day delay and vitrified embryos after one‐step warming and dilution was evaluated at the experimental facility. Although the resulting farrowing rate was 42.9%, the survival rate was 6.4%. In Experiment 3, Ns‐ET was conducted using V/T/W embryos at four commercial farms, where piglets derived from them were produced. When artificial insemination was conducted prior to Ns‐ET, the farrowing and survival rates obtained using V/T/W embryos were 75.0%, and 21.3%, respectively. These results show that Ns‐ET of V/T/W embryos using this protocol would be feasible for piglet production at farms. |
| Databáze: | OpenAIRE |
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