CD13 orients the apical-basal polarity axis necessary for lumen formation
| Autor: | Claire M. Brown, Luke McCaffrey, Li-Ting Wang, Abira Rajah |
|---|---|
| Jazyk: | angličtina |
| Rok vydání: | 2021 |
| Předmět: |
Polarity (physics)
Endosome Science General Physics and Astronomy Apicobasal polarity Endosomes CD13 Antigens General Biochemistry Genetics and Molecular Biology Epithelium Article 03 medical and health sciences 0302 clinical medicine Protein Domains Humans 030304 developmental biology Epithelial polarity Adaptor Proteins Signal Transducing 0303 health sciences Multidisciplinary Chemistry Cell Membrane Cell Polarity Membrane Proteins Epithelial Cells General Chemistry Apical membrane Transmembrane protein Endocytosis Cell biology rab GTP-Binding Proteins 030220 oncology & carcinogenesis Apical complex Caco-2 Cells Intracellular Lumen (unit) |
| Zdroj: | Nature Communications, Vol 12, Iss 1, Pp 1-15 (2021) Nature Communications |
| ISSN: | 2041-1723 |
| Popis: | Polarized epithelial cells can organize into complex structures with a characteristic central lumen. Lumen formation requires that cells coordinately orient their polarity axis so that the basolateral domain is on the outside and apical domain inside epithelial structures. Here we show that the transmembrane aminopeptidase, CD13, is a key determinant of epithelial polarity orientation. CD13 localizes to the apical membrane and associates with an apical complex with Par6. CD13-deficient cells display inverted polarity in which apical proteins are retained on the outer cell periphery and fail to accumulate at an intercellular apical initiation site. Here we show that CD13 is required to couple apical protein cargo to Rab11-endosomes and for capture of endosomes at the apical initiation site. This role in polarity utilizes the short intracellular domain but is independent of CD13 peptidase activity. Epithelial cells that organise into structures that contain a lumen are polarised. Here, the authors show that the short intracellular domain of transmembrane protein CD13 is required to capture endosomes at the apical site and is required for the polarisation of cells. |
| Databáze: | OpenAIRE |
| Externí odkaz: |
|
Full text from SpringerLink