Novel Precursors of Fluorescent Dyes. 1. Interaction of the Dyes with Model Phospholipid in Monolayers
| Autor: | Sergei Yu. Zaitsev, Dietmar Möbius, Ilia S. Zaitsev, Daria O. Solovyeva, Mikhail N. Shaposhnikov |
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| Rok vydání: | 2013 |
| Předmět: |
Models
Molecular Light Rhodamines Chemistry Air Phosphatidylethanolamines Biological objects Biophysics Phospholipid Water Biological membrane Cell Biology General Medicine Photochemistry Biochemistry Fluorescence Rhodamine chemistry.chemical_compound Membrane Monolayer Organic chemistry Phospholipids Fluorescent Dyes |
| Zdroj: | Cell Biochemistry and Biophysics |
| ISSN: | 1559-0283 1085-9195 |
| DOI: | 10.1007/s12013-013-9668-7 |
| Popis: | Photoactivated ("caged") fluorescent dyes are modern tools for structure and function studies of cell membranes and subcellular organelles. Recently synthesized precursors of rhodamine fluorescent dyes (abbreviations PFD813 and PFD814) important for microscopic probing of biological objects have been studied in solution. In order to characterize the behavior at interfaces, monolayers of PFD813 and PFD814 on water have been formed and investigated. The interactions of these precursors with the biomembrane component dimyristoylphosphatidylethanolamine in monolayers at the air-water interface and after transfer to glass plates have been studied by measuring monolayer parameters and spectroscopic properties before and after photo-chemical formation of the fluorescent rhodamine dyes Rho813 and Rho814, respectively. |
| Databáze: | OpenAIRE |
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