Phase Diagram for a Lysyl‐Phosphatidylglycerol Analogue in Biomimetic Mixed Monolayers with Phosphatidylglycerol: Insights into the Tunable Properties of Bacterial Membranes

Autor: Thomas Guttenberg, Hala Youssef, Helene Marbach, Chen Shen, Gema Vizcay-Barrena, Christian Wölk, Richard D. Harvey, Gerald Brezesinski
Rok vydání: 2020
Předmět:
Staphylococcus aureus
Langmuir
Surface Properties
Lipid Bilayers
Antimicrobial peptides
02 engineering and technology
010402 general chemistry
Models
Biological
01 natural sciences
Biophysical Phenomena
Membrane Lipids
chemistry.chemical_compound
Biomimetic Materials
Monolayer
antimicrobial resistance
Surface charge
Physical and Theoretical Chemistry
aminoacyl lipids
Phosphatidylglycerol
biology
Chemistry
Communication
Lysine
technology
industry
and agriculture
Membranes
Artificial
Phosphatidylglycerols
021001 nanoscience & nanotechnology
biology.organism_classification
Communications
Atomic and Molecular Physics
and Optics
Anti-Bacterial Agents
0104 chemical sciences
Membrane
ddc:540
Biophysics
lipids (amino acids
peptides
and proteins)
0210 nano-technology
membrane biophysics
Membrane biophysics
catanionic monolayers
Bacteria
Zdroj: ChemPhysChem 21(8), 702-706 (2020). doi:10.1002/cphc.202000026
ChemPhysChem
Chemphyschem
ISSN: 1439-7641
1439-4235
Popis: ChemPhysChem 21(8), 702 - 706 (2020). doi:10.1002/cphc.202000026
Ion pairing between the major phospholipids of the Staphylococcus aureus plasma membrane (phosphatidylglycerol – PG and lysyl‐phosphatidylglycerol – LPG) confers resistance to antimicrobial peptides and other antibiotics. We developed 3adLPG, a stable synthetic analogue which can substitute for the highy‐labile native LPG, in biophysical experiments examining the membrane‐protecting role of lipid ion pairing, in S. aureus and other important bacteria. Here we examine the surface charge and lipid packing characteristics of synthetic biomimetic mixtures of DPPG and DP3adLPG in Langmuir monolayers, using a combination of complementary surface‐probing techniques such as infrared reflection‐absorption spectroscopy and grazing‐incidence x‐ray diffraction. The resultant phase diagram for the ion paired lipids sheds light on the mixing behavior of lipids in monolayer models of resistant phenotype bacterial membranes, and provides a platform for future biophysical studies.
Published by Wiley-VCH Verl., Weinheim
Databáze: OpenAIRE
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