Investigation of In Vitro Endocrine Activities of Microcystis and Planktothrix Cyanobacterial Strains

Autor: Lisa Connolly, Lada Ivanova, Vittoria Mallia, Emma Harper, Silvio Uhlig, Gunnar Sundstøl Eriksen
Jazyk: angličtina
Předmět:
Cyanobacteria
Health
Toxicology and Mutagenesis
lcsh:Medicine
Endocrine Disruptors
010501 environmental sciences
Toxicology
01 natural sciences
Planktothrix
cyanobacteria
Biotransformation
Genes
Reporter
estrogenic
Cell Line
Transformed
chemistry.chemical_classification
endocrine disruptor
0303 health sciences
Estradiol
Microcystis
3. Good health
Receptors
Estrogen
Biochemistry
Endocrine disruptor
Receptors
Androgen
Microsomes
Liver
microcystin
medicine.drug_class
Bacterial Toxins
Microcystin
Biology
Risk Assessment
Article
03 medical and health sciences
Receptors
Glucocorticoid
SDG 3 - Good Health and Well-being
medicine
Humans
Endocrine system
030304 developmental biology
0105 earth and related environmental sciences
lcsh:R
Estrogens
biology.organism_classification
reporter-gene assay
Kinetics
chemistry
Estrogen
Zdroj: Toxins
Toxins, Vol 12, Iss 228, p 228 (2020)
Mallia, V, Ivanova, L, Eriksen, G S, Harper, E, Connolly, L & Uhlig, S 2020, ' Investigation of In Vitro Endocrine Activities of Microcystis and Planktothrix Cyanobacterial Strains ', Toxins, vol. 12, no. 4 . https://doi.org/10.3390/toxins12040228
Volume 12
Issue 4
ISSN: 2072-6651
DOI: 10.3390/toxins12040228
Popis: Cyanobacteria are cosmopolitan photosynthetic prokaryotes that can form dense accumulations in aquatic environments. They are able to produce many bioactive metabolites, some of which are potentially endocrine disrupting compounds, i.e., compounds that interfere with the hormonal systems of animals and humans. Endocrine disruptors represent potential risks to both environmental and human health, making them a global challenge. The aim of this study was to investigate the potential endocrine disrupting activities with emphasis on estrogenic effects of extracts from cultures of Microcystis or Planktothrix species. We also assessed the possible role of microcystins, some of the most studied cyanobacterial toxins, and thus included both microcystin-producing and non-producing strains. Extracts from 26 cyanobacterial cultures were initially screened in estrogen-, androgen-, and glucocorticoid-responsive reporter-gene assays (RGAs) in order to identify endocrine disruption at the level of nuclear receptor transcriptional activity. Extracts from selected strains were tested repeatedly in the estrogen-responsive RGAs, but the observed estrogen agonist and antagonist activity was minor and similar to that of the cyanobacteria growth medium control. We thus focused on another, non-receptor mediated mechanism of action, and studied the 17&beta
estradiol (natural estrogen hormone) biotransformation in human liver microsomes in the presence or absence of microcystin-LR (MC-LR), or an extract from the MC-LR producing M. aeruginosa PCC7806 strain. Our results show a modulating effect on the estradiol biotransformation. Thus, while 2-hydroxylation was significantly decreased following co-incubation of 17&beta
estradiol with MC-LR or M. aeruginosa PCC7806 extract, the relative concentration of estrone was increased.
Databáze: OpenAIRE
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