Corticosteroids induce the differential expression of TGFbeta isoforms, receptors and signaling in the gastric mucosa of suckling rats
| Autor: | Patrícia Gama, Eliana Parisi Alvares, Daniela Ogias, Bianca Bitencourt |
|---|---|
| Rok vydání: | 2005 |
| Předmět: |
medicine.medical_specialty
Hydrocortisone Physiology Clinical Biochemistry Smad2 Protein Biology Biochemistry Cellular and Molecular Neuroscience Endocrinology Pregnancy Transforming Growth Factor beta Internal medicine medicine Gastric mucosa Animals Protein Isoforms Rats Wistar Receptor Transforming growth factor beta Animals Suckling Rats Blot medicine.anatomical_structure Gastric Mucosa biology.protein Phosphorylation Female Signal transduction Receptors Transforming Growth Factor beta Glucocorticoid medicine.drug Hormone Signal Transduction |
| Zdroj: | Regulatory peptides. 135(1-2) |
| ISSN: | 0167-0115 |
| Popis: | Glucocorticoids inhibit the cell proliferation in the gastric epithelium, and induce differentiation, migration and death. The mechanism by which these effects are triggered and controlled is still discussed and can involve the transcription and activation of transforming growth factor beta (TGFbeta). The present study was conducted to evaluate the effect of hydrocortisone short-term treatment on tissue level and distribution of TGFbeta isoforms, receptors and signaling through Smad2/3. To achieve that, 18-day-old rats were injected with hydrocortisone (50 mg/Kg b.wt.) for 0, 1 and 3 h. The stomachs were collected and processed for immunohistochemistry and western blotting. We observed that the treatment for 3 h increased the number of labeled epithelial cells for TGFbeta1 (p < 0.05), decreased the distribution of TGFbeta2 (p < 0.05) and did not alter TGFbeta3, TbetaRI and TbetaRII status. The levels of TGFbeta1 and receptors were checked by western blotting and results corroborate the immunodetection. We also found that phosphorylation of Smad2/3 into Smad2P increased after 3 h (p < 0.05), indicating that the high level TGFbeta1 was active on the cells. We suggest that glucocorticoids differentially regulate the expression of TGFbeta isoforms, receptors and signaling, and so TGFbeta1 might be involved in the inhibitory pathway triggered by the hormone. |
| Databáze: | OpenAIRE |
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