Evaluation of apoptotic caspase levels in estimation of the wound age
| Autor: | Atiye Seda Yar Saglam, Taner Akar, Adnan Menevse, Ebru Alp, Ece Konac, Pınar Uyar Göçün |
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| Přispěvatelé: | Fakülteler, Tıp Fakültesi, Temel Tıp Bilimleri Bölümü, Tıbbi Biyoloji Ana Bilim Dalı, Alp, Ebru |
| Rok vydání: | 2017 |
| Předmět: |
0301 basic medicine
Forensic Science integumentary system biology business.industry Biochemistry (medical) Clinical Biochemistry Real Time PCR Immunohistochemistry Wound Age Estimation Biochemistry 03 medical and health sciences 030104 developmental biology 0302 clinical medicine Real-time polymerase chain reaction Apoptosis Cancer research biology.protein Medicine 030216 legal & forensic medicine business Molecular Biology Caspase |
| Zdroj: | Turkish Journal of Biochemistry. 43:126-133 |
| ISSN: | 1303-829X |
| Popis: | Objectives:We aimed to investigate the potential use of the expression of apoptotic signaling pathway genes of rat in skin wound age estimation.Material and methods:For this purpose, we formed cutting tool injuries using a scalpel in an experimental model. Then, we assessed Caspase 3, 8 and 9 mRNA levels by using quantitative real-time PCR and protein levels by using immunohistochemistry in rat skin wounds. In addition, we used TUNEL assay to detect apoptotic cells.Results:We observed that Caspase 3 mRNA level significantly increased (2.1±0.4 folds) on day 3 (pConclusion:Our results suggest that time-dependent features of apoptotic factors might offer a potential tool in estimating wound age. |
| Databáze: | OpenAIRE |
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