Glutathione S-Transferase Iso-Enzymes in Perfusate From Pumped Kidneys Are Associated With Delayed Graft Function
| Autor: | Francis L. Weng, Bernd Schröppel, Peter P. Reese, Gang Han, Rick D. Hasz, Isaac E. Hall, Ronik S. Bhangoo, Mona D. Doshi, Kwangik Hong, Chirag R. Parikh, Haiqun Lin, Michael J. Goldstein |
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| Rok vydání: | 2014 |
| Předmět: |
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medicine.medical_specialty medicine.medical_treatment Urology Delayed Graft Function Renal function Enzyme-Linked Immunosorbent Assay Kidney Function Tests Article Postoperative Complications Risk Factors medicine Humans Immunology and Allergy Pharmacology (medical) Prospective Studies Kidney transplantation Dialysis Glutathione Transferase Transplantation Kidney Machine perfusion biology business.industry Middle Aged Prognosis medicine.disease Kidney Transplantation Surgery Isoenzymes Perfusion medicine.anatomical_structure Glutathione S-transferase Glutathione S-Transferase pi biology.protein Kidney Failure Chronic Female business Biomarkers Follow-Up Studies Glomerular Filtration Rate |
| Zdroj: | American Journal of Transplantation. 14:886-896 |
| ISSN: | 1600-6135 |
| Popis: | Accurate and reliable assessment tools are needed in transplantation. The objective of this prospective, multicenter study was to determine the associations of the alpha and pi iso-enzymes of glutathione S-transferase (GST), measured from perfusate solution at the start and end (base and post) of kidney allograft machine perfusion, with subsequent delayed graft function (DGF). We also compared GST iso-enzyme perfusate levels from discarded versus transplanted kidneys. A total of 428 kidneys were linked to outcomes as recorded by the United Network of Organ Sharing. DGF, defined as any dialysis in the first week of transplant, occurred in 141 recipients (32%). Alpha and pi-GST levels significantly increased during machine perfusion. The adjusted relative risks (95% confidence interval) of DGF with each log-unit increase in base and post pi-GST were 1.14 (1.0-1.28) and 1.33 (1.02-1.72), respectively. Alpha-GST was not independently associated with DGF. There were no significant differences in GST values between discarded and transplanted kidneys, though renal resistance was significantly higher in discarded kidneys. We found pi-GST at the end of machine perfusion to be independently associated with DGF. Further studies should elucidate the utility of GST for identifying injured kidneys with regard to organ allocation, discard and recipient management decisions. |
| Databáze: | OpenAIRE |
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