Correction to: Genome-wide profiling of DNA methylation and gene expression identifies candidate genes for human diabetic neuropathy
| Autor: | Stephanie Eid, Junguk Hur, Eva L. Feldman, Crystal Pacut, Sarah Elzinga, Kai Guo |
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| Rok vydání: | 2020 |
| Předmět: |
Male
Candidate gene Diabetic neuropathy Genome wide profiling Computational biology Biology Epigenesis Genetic Diabetic Neuropathies Double-Blind Method Gene expression Genetics medicine Cluster Analysis Humans Molecular Biology Genetics (clinical) Gene Expression Profiling Correction DNA Methylation Middle Aged medicine.disease Human genetics Gene Expression Regulation DNA methylation Female Developmental Biology |
| Zdroj: | Clinical Epigenetics |
| ISSN: | 1868-7083 1868-7075 |
| DOI: | 10.1186/s13148-020-00922-5 |
| Popis: | Diabetic peripheral neuropathy (DPN) is the most common complication of type 2 diabetes (T2D). Although the cellular and molecular mechanisms of DPN are poorly understood, we and others have shown that altered gene expression and DNA methylation are implicated in disease pathogenesis. However, how DNA methylation might functionally impact gene expression and contribute to nerve damage remains unclear. Here, we analyzed genome-wide transcriptomic and methylomic profiles of sural nerves from T2D patients with DPN.Unbiased clustering of transcriptomics data separated samples into groups, which correlated with HbA1c levels. Accordingly, we found 998 differentially expressed genes (DEGs) and 929 differentially methylated genes (DMGs) between the groups with the highest and lowest HbA1c levels. Functional enrichment analysis revealed that DEGs and DMGs were enriched for pathways known to play a role in DPN, including those related to the immune system, extracellular matrix (ECM), and axon guidance. To understand the interaction between the transcriptome and methylome in DPN, we performed an integrated analysis of the overlapping genes between DEGs and DMGs. Integrated functional and network analysis identified genes and pathways modulating functions such as immune response, ECM regulation, and PI3K-Akt signaling.These results suggest for the first time that DNA methylation is a mechanism regulating gene expression in DPN. Overall, DPN patients with high HbA1c have distinct alterations in sural nerve DNA methylome and transcriptome, suggesting that optimal glycemic control in DPN patients is an important factor in maintaining epigenetic homeostasis and nerve function. |
| Databáze: | OpenAIRE |
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