Topoisomerase II is regulated by translationally controlled tumor protein for cell survival during organ growth in Drosophila
| Autor: | Robert Amson, Stephanie B. Telerman, Kwang-Wook Choi, Jung-Wan Mok, Adam Telerman, Dae-Wook Yang |
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| Přispěvatelé: | Choi, Kwang-Wook [0000-0002-8997-3065], Apollo - University of Cambridge Repository |
| Rok vydání: | 2021 |
| Předmět: |
Male
Cancer Research 631/136/83 Protein family Cell Survival Organogenesis Immunology Green Fluorescent Proteins 631/136/2060 medicine.disease_cause 14/1 Cellular and Molecular Neuroscience Cell growth RNA interference Translationally-controlled tumor protein medicine Animals Drosophila Proteins Wings Animal 14/19 biology QH573-671 Topoisomerase article Intracellular Signaling Peptides and Proteins Epistasis Genetic Cell Biology Phenotype Cell biology 64/24 DNA Topoisomerases Type II Drosophila melanogaster 13/51 biology.protein Female RNA Interference Carcinogenesis Cytology |
| Zdroj: | Cell Death and Disease, Vol 12, Iss 9, Pp 1-10 (2021) Cell Death & Disease |
| Popis: | Regulation of cell survival is critical for organ development. Translationally controlled tumor protein (TCTP) is a conserved protein family implicated in the control of cell survival during normal development and tumorigenesis. Previously, we have identified a human Topoisomerase II (TOP2) as a TCTP partner, but its role in vivo has been unknown. To determine the significance of this interaction, we examined their roles in developing Drosophila organs. Top2 RNAi in the wing disc leads to tissue reduction and caspase activation, indicating the essential role of Top2 for cell survival. Top2 RNAi in the eye disc also causes loss of eye and head tissues. Tctp RNAi enhances the phenotypes of Top2 RNAi. The depletion of Tctp reduces Top2 levels in the wing disc and vice versa. Wing size is reduced by Top2 overexpression, implying that proper regulation of Top2 level is important for normal organ development. The wing phenotype of Tctp RNAi is partially suppressed by Top2 overexpression. This study suggests that mutual regulation of Tctp and Top2 protein levels is critical for cell survival during organ development. |
| Databáze: | OpenAIRE |
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