tRNA 2 Thr Complements Temperature Sensitivity Caused by Null Mutations in the htrB Gene in Escherichia coli
| Autor: | Simon Goto, Yoshio Mohri, Hachiro Inokuchi, Kenji Nakahigashi |
|---|---|
| Rok vydání: | 2003 |
| Předmět: |
Genomics and Proteomics
Mutant Biology medicine.disease_cause Microbiology Bacterial Proteins Anticodon Escherichia coli medicine Protein biosynthesis Molecular Biology Gene RNA Transfer Thr chemistry.chemical_classification Genetics Mutation Escherichia coli Proteins Temperature Phenotype Lipid A Enzyme chemistry Biochemistry Transfer RNA Acyltransferases |
| Zdroj: | Journal of Bacteriology. 185:1726-1729 |
| ISSN: | 1098-5530 0021-9193 |
| DOI: | 10.1128/jb.185.5.1726-1729.2003 |
| Popis: | According to the wobble rule, tRNA2Thr is nonessential for protein synthesis, because the codon (ACG) that is recognized by tRNA2Thr is also recognized by tRNA4Thr. In order to investigate the reason that this nonessential tRNA nevertheless exists in Escherichia coli , we attempted to isolate tRNA2Thr-requiring mutants. Using strain JM101F − , which lacks the gene for tRNA2Thr, we succeeded in isolating two temperature-sensitive mutants whose temperature sensitivity was complemented by introduction of the gene for tRNA2Thr. These mutants had a mutation in the htrB gene, whose product is an enzyme involved in lipid A biosynthesis. Although it is known that some null mutations in the htrB gene give a temperature-sensitive phenotype, our mutants exhibited tighter temperature sensitivity. We discuss a possible mechanism for the requirement for tRNA2Thr. |
| Databáze: | OpenAIRE |
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