Widespread Enhancer Dememorization and Promoter Priming during Parental-to-Zygotic Transition
| Autor: | Wen-Hao Zhang, Qiujun Wang, Xiaotong Wu, Bingjie Zhang, Weimin Shen, Qingrui Sun, Wei Xie, Anming Meng, Kaili Liu, Yu Zhang, Yuanyuan Li |
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| Rok vydání: | 2018 |
| Předmět: |
0301 basic medicine
Epigenomics Male Transcription Genetic Zygote Biology Regulatory Sequences Nucleic Acid Epigenesis Genetic Histones 03 medical and health sciences Animals Epigenetics Enhancer Promoter Regions Genetic Molecular Biology Zebrafish Histone Acetyltransferases Genome Gene Expression Regulation Developmental Acetylation Cell Biology Epigenome DNA Methylation Zebrafish Proteins biology.organism_classification Spermatozoa Cell biology Histone Code 030104 developmental biology Histone biology.protein Oocytes Maternal to zygotic transition Reprogramming Protein Processing Post-Translational |
| Zdroj: | Molecular cell. 72(4) |
| ISSN: | 1097-4164 |
| Popis: | The epigenome plays critical roles in controlling gene expression and development. However, how the parental epigenomes transit to the zygotic epigenome in early development remains elusive. Here we show that parental-to-zygotic transition in zebrafish involves extensive erasure of parental epigenetic memory, starting with methylating gametic enhancers. Surprisingly, this occurs even prior to fertilization for sperm. Both parental enhancers lose histone marks by the 4-cell stage, and zygotic enhancers are not activated until around zygotic genome activation (ZGA). By contrast, many promoters remain hypomethylated and, unexpectedly, acquire histone acetylation before ZGA at as early as the 4-cell stage. They then resolve into either activated or repressed promoters upon ZGA. Maternal depletion of histone acetyltransferases results in aberrant ZGA and early embryonic lethality. Finally, such reprogramming is largely driven by maternal factors, with zygotic products mainly contributing to embryonic enhancer activation. These data reveal widespread enhancer dememorization and promoter priming during parental-to-zygotic transition. |
| Databáze: | OpenAIRE |
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