Applicability and robustness of the hen's egg test for analysis of micronucleus induction (HET-MN): results from an inter-laboratory trial
| Autor: | Thorsten Wolf, Klaus Rudolf Schroeder, Kerstin Reisinger, Norbert Dr Banduhn, Matthias Krauledat, Julia Scheel, Daniela Greywe, Jürgen Kreutz |
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| Rok vydání: | 2011 |
| Předmět: |
Chromatography
Micronucleus Tests Health Toxicology and Mutagenesis Eggs Alternatives to animal testing Reproducibility of Results medicine.disease_cause Toxicology chemistry.chemical_compound Clastogen chemistry In vivo Micronucleus test Genetics medicine Animals Inter-laboratory Micronucleus Isopropyl myristate Chickens Genotoxicity Mutagens |
| Zdroj: | Mutation research. 747(1) |
| ISSN: | 0027-5107 |
| Popis: | The hen's egg test for analysis of micronucleus formation (HET-MN) was developed several years ago to provide an alternative test system to the in vivo micronucleus test. In order to assess its applicability and robustness, a study was carried out at the University of Osnabrueck (lab A) and at the laboratories of Henkel AG & Co. KGaA (lab B). Following transfer of the method to lab B, a range of test substances that had been pre-tested at lab A, were tested at Henkel: the genotoxins cyclophosphamide, dimethylbenz(a)anthracene, methotrexate, acrylamide, azorubin, N-nitroso-dimethylamine and the non-genotoxins, orange G and isopropyl myristate. In a second phase, additional compounds with known in vivo properties were examined in both labs: the non-genotoxin, ampicillin, the “irrelevant” positives, isophorone and 2,4-dichlorophenol (“irrelevant” means positive in standard in vitro tests, but negative in vivo), the clastogen p-chloroaniline, and the aneugens carbendazim and vinorelbine. All substances were correctly predicted in both labs with respect to their in vivo genotoxic properties, indicating that the HET-MN may have an improved predictivity compared with current standard in vitro test systems. The results support the promising role of the HET-MN assay as a supplement to existing test batteries. |
| Databáze: | OpenAIRE |
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