Purification of a lectin from Canavalia ensiformis using PEG–citrate aqueous two-phase system
| Autor: | Maria G. Carneiro-da-Cunha, Paulo A.G. Soares, Ana Lúcia Figueiredo Porto, Maria Tereza dos Santos Correia, Tatiana Souza Porto, Cynthia O. Nascimento |
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| Rok vydání: | 2011 |
| Předmět: |
Clinical Biochemistry
Chemical Fractionation Biochemistry Citric Acid Polyethylene Glycols Analytical Chemistry PEG ratio Concanavalin A Analysis of Variance Aqueous solution Chromatography Downstream processing biology Aqueous medium Plant Extracts Chemistry Aqueous two-phase system Lectin Cell Biology General Medicine Hydrogen-Ion Concentration biology.organism_classification Molecular Weight Canavalia Canavalia ensiformis Seeds biology.protein Electrophoresis Polyacrylamide Gel |
| Zdroj: | Journal of Chromatography B. 879:457-460 |
| ISSN: | 1570-0232 |
| DOI: | 10.1016/j.jchromb.2010.12.030 |
| Popis: | A PEG/citrate aqueous two-phase system was tested in the partition of commercial Concanavalin A (Con A) and subsequently applied to the extraction and purification of Con A from the crude extract of Canavalia ensiformis seeds. Con A was successfully extracted to the bottom phase of a system composed of 22% (w/w) PEG8000 and 12% (w/w) citrate at pH 6.0. The obtained purification factor was 11.5 without any loss in the hemagglutinating activity. The purity of extracted lectin was confirmed by SDS-PAGE analysis. |
| Databáze: | OpenAIRE |
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