Enzyme immobilization onto the nanomaterials: Application in enzyme stability and prodrug-activated cancer therapy
| Autor: | Abbas Salihi, Abdulkarim Yasin Karim, Rizwan Hasan Khan, Ali Akbar Saboury, Mojtaba Falahati, Sara Haji Hosseinali, Nadir Mustafa Qadir Nanakali, Amir Jouya Talaei, Bing Yan, Majid Sharifi, Falah Mohammad Aziz, Mohammad Javad Sohrabi, Anwarul Hasan, Pegah Hashemi Kani |
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| Rok vydání: | 2020 |
| Předmět: |
Immobilized enzyme
Cancer therapy Nanoparticle Nanotechnology 02 engineering and technology Biochemistry Nanomaterials 03 medical and health sciences Structural Biology Neoplasms Enzyme Stability Animals Humans Prodrugs Molecular Biology 030304 developmental biology chemistry.chemical_classification 0303 health sciences Biomolecule technology industry and agriculture General Medicine Prodrug Enzymes Immobilized 021001 nanoscience & nanotechnology Nanostructures Cancer treatment Enzyme chemistry 0210 nano-technology |
| Zdroj: | International Journal of Biological Macromolecules. 143:665-676 |
| ISSN: | 0141-8130 |
| DOI: | 10.1016/j.ijbiomac.2019.12.064 |
| Popis: | Nanoparticles (NPs) have been widely used for immobilization of wide ranges of enzymes. However, the stabilization of enzymes on NPs is a major challenge, crucial for regulating enzymatic activity and their medical applications. To overcome these challenges, it is necessary to explore how enzymes attach to nanomaterials and their properties are affected by such interactions. In this review we present an overview on the different strategies of the enzyme immobilization into the NPs and their corresponding stability against temperature and pH. The effects of surface charge, particle size, morphology, and aggregation of NPs on the stability of immobilized enzymes were summarized. The activity of immobilized enzyme into the NPs was reviewed to disclose more detail regarding the interaction of biomolecules with NPs. The combination of enzyme immobilization with prodrugs was also reviewed as a promising approach for biomedical application of enzyme in cancer therapy. Finally, the current challenges and future applications of NPs in enzyme immobilization and the utilization of immobilized enzyme toward prodrug activation in cytoplasm of cancer cells were presented. In conclusion, this review may pave the way for providing a perspective on development to the industrial and clinical translation of immobilized enzymes. |
| Databáze: | OpenAIRE |
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