Regulation of a mammalian gene bearing a CpG island promoter and a distal enhancer
| Autor: | Mark Ptashne, Georgina Berrozpe, Gene O. Bryant, Katherine Warpinski |
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| Rok vydání: | 2013 |
| Předmět: |
Molecular Sequence Data
Repressor Biology General Biochemistry Genetics and Molecular Biology Mice Transcription (biology) Yeasts Nucleosome Animals Mast Cells RNA Messenger Enhancer Mammalian gene Promoter Regions Genetic lcsh:QH301-705.5 Gene Alleles Base Sequence DNA Methylation Molecular biology Yeast Nucleosomes Proto-Oncogene Proteins c-kit lcsh:Biology (General) CpG site CpG Islands Myogenin |
| Zdroj: | Cell Reports, Vol 4, Iss 3, Pp 445-453 (2013) |
| ISSN: | 2211-1247 |
| Popis: | Summary A quantitative nucleosome occupancy assay revealed rules for nucleosome disposition in yeast and showed how disposition affects regulation of the GAL genes. Here, we show how those findings apply to the control of Kit , a mammalian gene. The Kit promoter lies in a CpG island, and its enhancer (active in mast cells) lies some 150 kb upstream. Nucleosomes form with especially high avidities at the Kit promoter, a reaction that, we surmise, ensures extremely low basal expression. In mast cells, transcriptional activators displace nucleosomes that are less tightly formed at the Kit enhancer. In turn, the active enhancer replaces a single Kit promoter nucleosome with the transcriptional machinery, thereby inducing transcription over 1,000-fold. As at the yeast GAL genes, the inhibitory effects of nucleosomes facilitate high factors of induction by mammalian activators working in the absence of specific repressors. |
| Databáze: | OpenAIRE |
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