Polypeptide and glycosaminoglycan polysaccharide as stabilizing polymers in nanocrystals for a safe ocular hypotensive effect
| Autor: | Rihab Osman, Marwa Donia, Gehanne A.S. Awad, Nahed D. Mortada |
|---|---|
| Rok vydání: | 2020 |
| Předmět: |
Biocompatibility
Sonication Biological Availability Biocompatible Materials 02 engineering and technology Eye Biochemistry Polyvinyl alcohol 03 medical and health sciences chemistry.chemical_compound Crystallinity Differential scanning calorimetry Structural Biology Lecithins Animals Molecular Biology Glycosaminoglycans 030304 developmental biology Drug Carriers 0303 health sciences Glaucoma General Medicine 021001 nanoscience & nanotechnology Acetazolamide Solvent chemistry Polyvinyl Alcohol Spray drying Nanoparticles Draize test Rabbits Soybeans Peptides 0210 nano-technology Nuclear chemistry |
| Zdroj: | International Journal of Biological Macromolecules. 162:1699-1710 |
| ISSN: | 0141-8130 |
| Popis: | Improved ocular delivery of a poorly soluble anti-glaucoma drug, acetazolamide (ACZ), in a stable nanosuspension (NS) was the main target of the study. The anionic polypeptide, poly-γ-glutamic acid (PG) and the glycosaminoglycan, hyaluronic acid, were used to stabilize ACZ-NS prepared using the antisolvent precipitation (AS-PT) coupled with sonication technique. To endue in site biocompatibility with high tolerability, soya lecithin (SL) phospholipid has been also combined with polyvinyl alcohol (PVA). NS with uniform PS in the range 100–300 nm, high ζ > ±20 mV, and enhanced saturation solubility were produced. Targeting solvent removal with control on future particle growth, post-production processing of NS was done using spray drying. The carriers' composition and amount relative to ACZ-NS were optimized to allow for the production of a redispersible dry crystalline powder. Particles crystallinity was confirmed using X-ray powder diffraction (XRPD) and differential scanning calorimetry (DSC) in liquid and spray dried NS. The modified Draize test proved the safety and tolerability following application to rabbit eyes accompanying an efficient ocular hypotensive activity using a steroid glaucoma model. |
| Databáze: | OpenAIRE |
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