Identification of Aspergillus fumigatus Surface Components That Mediate Interaction of Conidia and Hyphae With Human Platelets
| Autor: | Jean-Paul Latgé, Sven Krappmann, Thierry Fontaine, Cornelia Lass-Flörl, Gerhard Blum, Hanna Jeckström, Cornelia Speth, Magdalena Hagleitner, Thorsten Heinekamp, Günter Rambach, Günter Weigel, Kristian Pfaller, Philipp Würtinger, Jürgen Löffler |
|---|---|
| Přispěvatelé: | Innsbruck Medical University [Austria] (IMU), Aspergillus, Institut Pasteur [Paris], Leibniz Institute for Natural Product Research and Infection Biology (Hans Knoell Institute), University Hospital Innsbruck, Friedrich-Alexander Universität Erlangen-Nürnberg (FAU), University Hospital of Würzburg, This work was supported by the Fonds zur Förderung der Wissenschaftlichen Forschung (project P26117-B20)., Innsbruck Medical University = Medizinische Universität Innsbruck (IMU), Institut Pasteur [Paris] (IP) |
| Rok vydání: | 2014 |
| Předmět: |
beta-Glucans
Galactosaminogalactan MESH: Flow Cytometry Chitin MESH: Chitin Aspergillus fumigatus Melanin chemistry.chemical_compound MESH: Melanins Medizinische Fakultät Immunology and Allergy Platelet skin and connective tissue diseases innate immunity MESH: Blood Platelets [SDV.MP.MYC]Life Sciences [q-bio]/Microbiology and Parasitology/Mycology 0303 health sciences biology MESH: beta-Glucans Spores Fungal Flow Cytometry galactosaminogalactan melanin Infectious Diseases platelets Antigens Surface MESH: Fungal Proteins MESH: Immunity Innate MESH: Aspergillus fumigatus Blood Platelets hydrophobin Hypha Hydrophobin fungal surface MESH: Antigens Surface Virulence Factors Hyphae Microbiology Fungal Proteins 03 medical and health sciences MESH: Hyphae MESH: Spores Fungal Polysaccharides MESH: Platelet Activation Aspergillosis Humans Platelet activation MESH: Aspergillosis ddc:610 030304 developmental biology MESH: Virulence Factors Melanins invasive aspergillosis Innate immune system MESH: Humans 030306 microbiology fungi biology.organism_classification Platelet Activation Immunity Innate MESH: Polysaccharides chemistry |
| Zdroj: | Journal of Infectious Diseases Journal of Infectious Diseases, Oxford University Press (OUP), 2015, 212 (7), pp.1140-1149. ⟨10.1093/infdis/jiv191⟩ Journal of Infectious Diseases, 2015, 212 (7), pp.1140-1149. ⟨10.1093/infdis/jiv191⟩ |
| ISSN: | 1537-6613 0022-1899 |
| DOI: | 10.1093/infdis/jiv191⟩ |
| Popis: | International audience; Background. Platelets were recently identified as a part of innate immunity. They are activated by contact with Aspergillus fumigatus; putative consequences include antifungal defense but also thrombosis, excessive inflammation, and thrombocytopenia. We aimed to identify those fungal surface structures that mediate interaction with platelets.Methods. Human platelets were incubated with Aspergillus conidia and hyphae, isolated wall components, or fungal surface mutants. Interaction was visualized microscopically; activation was quantified by flow cytometry of specific markers.Results. The capacity of A. fumigatus conidia to activate platelets is at least partly due to melanin, because this effect can be mimicked with “melanin ghosts”; a mutant lacking melanin showed reduced platelet stimulating potency. In contrast, conidial hydrophobin masks relevant structures, because an A. fumigatus mutant lacking the hydrophobin protein induced stronger platelet activation than wild-type conidia. A. fumigatus hyphae also contain surface structures that interact with platelets. Wall proteins, galactomannan, chitin, and β-glucan are not the relevant hyphal components; instead, the recently identified fungal polysaccharide galactosaminogalactan potently triggered platelet activation.Conclusions. Conidial melanin and hydrophobin as well as hyphal galactosaminogalactan represent important pathogenicity factors that modulate platelet activity and thus might influence immune responses, inflammation, and thrombosis in infected patients. |
| Databáze: | OpenAIRE |
| Externí odkaz: |
|