Association of mRNA Levels of IL6, MMP-8, GSS in Saliva and Pyelonephritis in Children
| Autor: | Yoana Kiselova-Kaneva, Ayshe Salim, Sirma Angelova, Diana Ivanova, Stefan Peev |
|---|---|
| Jazyk: | angličtina |
| Rok vydání: | 2019 |
| Předmět: |
Male
Saliva medicine.medical_specialty Pharmaceutical Science Urine Matrix metalloproteinase Article Glutathione Synthase Analytical Chemistry lcsh:QD241-441 03 medical and health sciences 0302 clinical medicine mRNA levels lcsh:Organic chemistry children Internal medicine Drug Discovery medicine Humans In patient RNA Messenger GSS Physical and Theoretical Chemistry Child Messenger RNA salivary biomarkers of IL-6 business.industry Interleukin-6 Organic Chemistry 030206 dentistry Glutathione synthetase Reverse transcription polymerase chain reaction Endocrinology Matrix Metalloproteinase 8 Mrna level Chemistry (miscellaneous) 030220 oncology & carcinogenesis Child Preschool correlation Molecular Medicine pyelonephritis Female business MMP-8 Biomarkers |
| Zdroj: | Molecules Volume 25 Issue 1 Molecules, Vol 25, Iss 1, p 85 (2019) |
| ISSN: | 1420-3049 |
| DOI: | 10.3390/molecules25010085 |
| Popis: | Nowadays, saliva is a subject of growing scientific interest because of its definite advantages as diagnostic medium. The aim of our study was to investigate the diagnostic potential and reliability of messenger RNAs (mRNAs) of selected genes&mdash interleukin-6 (IL-6), matrix metalloproteinase-8 (MMP-8) and glutathione synthetase (GSS)&mdash as salivary markers in children with diagnosed pyelonephritis and to correlate their levels with typical urine para-clinical indicators of the disease. Analysis of the mRNA levels for IL-6, MMP-8 and GSS in 28 children hospitalized with the diagnosis of pyelonephritis was conducted applying the method of quantitative reverse transcription polymerase chain reaction (RT-qPCR). In the study group (n = 28), IL-6 mRNA levels demonstrated 64-fold increase (p < 0.001). MMP-8 and GSS mRNA levels were increased in 12 samples in patients with pyelonephritis 3.27 (p < 0.01) and 1.94 (p < 0.001) times, respectively. We found a strong and significant correlation (p < 0.001) between the investigated mRNA for IL-6 and MMP-8, IL-6 and GSS, MMP-8 and GSS. Moderate degree of correlation was established between IL-6 and the typical para-clinical indicator of leucocytes (0.43, p < 0.05) and between GSS and leucocytes (0.54, p < 0.01). Salivary IL-6, MMP-8 and GSS mRNA levels in combination with urine test analysis could be useful diagnostic tool for the very distributed disorder of pyelonephritis in childhood. |
| Databáze: | OpenAIRE |
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