Single-Nanoparticle ICPMS DNA Assay Based on Hybridization-Chain-Reaction-Mediated Spherical Nucleic Acid Assembly
| Autor: | Jian-Hui Jiang, Hao Tang, Ru-Qin Yu, Bang-Rui Li |
|---|---|
| Rok vydání: | 2020 |
| Předmět: |
chemistry.chemical_classification
Chromatography Biomolecule 010401 analytical chemistry Nanoparticle Nucleic Acid Hybridization DNA 010402 general chemistry Mass spectrometry 01 natural sciences Mass Spectrometry 0104 chemical sciences Analytical Chemistry chemistry.chemical_compound Nucleic acid thermodynamics chemistry Spherical nucleic acid Nucleic acid Nanoparticles Particle Size Inductively coupled plasma mass spectrometry |
| Zdroj: | Analytical chemistry. 92(3) |
| ISSN: | 1520-6882 |
| Popis: | The detection of nucleic acid is critical for clinic diagnostics. Single-nanoparticle inductively coupled plasma mass spectrometry (SP-ICPMS) has demonstrated unique advantages for nucleic acid detection. Here we report the development of a novel SP-ICPMS DNA assay based on a target-induced hybridization chain reaction to achieve controlled spherical nucleic acid assembly. The assembly process generated large gold nanoparticle aggregates, and the number of aggregates could be counted by SP-ICPMS, which was closely correlated to the concentration of the target DNA. This simple homogeneous assay could analyze DNA within the range of 5 fM to 10 pM with excellent selectivity and applicability for real sample analysis. It is envisioned that the developed approach might create a useful SP-ICPMS platform for biomolecule detection. |
| Databáze: | OpenAIRE |
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