Certification of the Mass Concentration of Creatine Kinase Isoenzyme 2 (CK-MB) in the Reference Material BCR 608
| Autor: | C. Profilis, Xavier Fuentes-Arderiu, Maribel Sánchez, E. Cusó, Mauro Panteghini, K. Hallermayer, Mogens Hørder, Ferruccio Ceriotti, F J Gella, Francesca Canalias, G. Schumann |
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| Jazyk: | angličtina |
| Rok vydání: | 2001 |
| Předmět: |
Creatine kinase-2
Certification Sodium Clinical Biochemistry chemistry.chemical_element Creatine chemistry.chemical_compound Reference Values Enzyme Stability Mole medicine Creatine Kinase MB Form Humans Mass concentration (chemistry) Creatine Kinase Chromatography medicine.diagnostic_test biology Myocardium Biochemistry (medical) General Medicine Reference Standards Chromatography Ion Exchange Standardization Isoenzymes chemistry Immunoassay Calibration biology.protein Electrophoresis Polyacrylamide Gel Creatine kinase Reference material Quantitative analysis (chemistry) Stock solution |
| Zdroj: | Sánchez, M, Gella, F-J, Profilis, C, Ceriotti, F, Cusó, E, Fuentes-Arderiu, X, Hallermayer, K, Hørder, M, Panteghini, M, Schumann, G & Canalias, F 2001, ' Certification of the Mass Concentration of Creatine Kinase Isoenzyme 2 (CK-MB) in the Reference Material BCR 608 ', Clinical Chemistry and Laboratory Medicine, vol. 39, no. 9, pp. 858-865 . https://doi.org/10.1515/CCLM.2001.142 |
| DOI: | 10.1515/CCLM.2001.142 |
| Popis: | We describe the certification of a mass concentration value in the already prepared creatine kinase-2 reference material (BCR 608). Creatine kinase-2 was purified from human heart. The purified enzyme was diluted in order to measure its protein concentration by the Doetsch method. A protein concentration value of 124.30+/-13.17 mg/l was assigned to the stock solution of purified creatine kinase-2. This stock solution was diluted in 25 mmol/l piperazine-N,N'-bis[2-ethanesulfonic acid] (PIPES) pH 7.2, containing 2 mmol/l ADP, 5 mmol/l 2-mercaptoethanol, 154 mmol/l sodium chloride and 50 g/l human albumin to obtain a stable liquid standard of known creatine kinase-2 mass concentration (80.36 microg/l). This standard was then used to recalculate the creatine kinase-2 mass concentration measured in the BCR 608 material by immunoassay. The mass concentration of creatine kinase-2 in samples of reconstituted BCR 608 was certified to be 93.30+/-9.65 microg/l. |
| Databáze: | OpenAIRE |
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