Vitamin D Inhibition of TRPV5 Expression During Osteoclast Differentiation
| Autor: | Yan Yuan, Jianchun Bian, Xi-Shuai Tong, Jianhong Gu, Xuezhong Liu, Yang Chen, Saihui Li, Tianhong Ma, Chuang Zhang, Wenyan Min, Zongping Liu |
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| Jazyk: | angličtina |
| Rok vydání: | 2019 |
| Předmět: |
TRPV5
Endocrinology Diabetes and Metabolism Carbonic anhydrase II 030209 endocrinology & metabolism Calcitriol receptor 03 medical and health sciences 0302 clinical medicine Osteoclast medicine Vitamin D and neurology Cathepsin K 030212 general & internal medicine Tartrate-resistant acid phosphatase biology business.industry RANKL Molecular biology Ca2+ medicine.anatomical_structure biology.protein 1 25(OH)2D3 business Research Article |
| Zdroj: | International Journal of Endocrinology and Metabolism |
| ISSN: | 1726-9148 1726-913X |
| Popis: | Background Vitamin D is an important steroid that can regulate bone metabolism including osteoclast (OC) differentiation. Transient receptor potential cation channel subfamily V member 5 (TRPV5), is a calcium channel protein involved in OC differentiation. However, the impact of vitamin D on TRPV5 expression during OC differentiation is not clear. Objectives To determine if 1,25-dihydroxyvitamin D3 (1,25(OH)2D3) regulates the expression of TRPV5 during OC differentiation. Methods Bone marrow mononuclear macrophage (BMMs) were induced to differentiate into OC with or without treatment with 10 nM 1,25(OH)2D3. The expression levels of vitamin D receptor (VDR) and TRPV5 were examined. The expression of several OC markers, including tartrate resistant acid phosphatase (TRAP), carbonic anhydrase II (Ca II), cathepsin K (CTSK), and vacuolar-type H+-ATPase (V-ATPase) were also detected. Results We found that the VDR was expressed in murine bone marrow-derived macrophages at the early stage of OC differentiation. TRPV5 expression was increased during OC differentiation, which was down-regulated by 1,25(OH)2D3 after a prolonged exposure. The 1,25(OH)2D3 and TRPV5 inhibitors inhibited OC differentiation. Conclusions 1,25(OH)2D3 can inhibit TRPV5 expression as well as TRPV5 inhibitors during OC differentiation. This suggests that 1,25(OH)2D3 may suppress OC differentiation by inhibiting TRPV5 expression. |
| Databáze: | OpenAIRE |
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