Serine protease activity in excretory–secretory products of Oestrus ovis (Diptera: Oestridae) larvae

Autor: Ph. Jacquiet, Guillaume Tabouret, Ph. Dorchies, L Bret-Bennis
Přispěvatelé: Interactions hôtes-agents pathogènes [Toulouse] (IHAP), Institut National de la Recherche Agronomique (INRA)-Ecole Nationale Vétérinaire de Toulouse (ENVT), Institut National Polytechnique (Toulouse) (Toulouse INP), Université Fédérale Toulouse Midi-Pyrénées-Université Fédérale Toulouse Midi-Pyrénées-Institut National Polytechnique (Toulouse) (Toulouse INP), Université Fédérale Toulouse Midi-Pyrénées-Université Fédérale Toulouse Midi-Pyrénées, Inconnu, ProdInra, Migration, Unité associée de Physiopathologie respiratoire des ruminants, Institut National de la Recherche Agronomique (INRA)
Rok vydání: 2003
Předmět:
[SDV]Life Sciences [q-bio]
Benzoylarginine Nitroanilide
IMMUNOLOGIE
Oestrus ovis
030308 mycology & parasitology
0302 clinical medicine
Ovis
ComputingMilieux_MISCELLANEOUS
0303 health sciences
biology
Hydrolysis
Serine Endopeptidases
General Medicine
Hydrogen-Ion Concentration
Trypsin
[SDV] Life Sciences [q-bio]
Biochemistry
Excretory system
Larva
Electrophoresis
Polyacrylamide Gel
Collagen
medicine.drug
Proteases
030231 tropical medicine
Sheep Diseases
Myiasis
03 medical and health sciences
medicine
Animals
Protease Inhibitors
Serum Albumin
Serine protease
[SDV.BA.MVSA]Life Sciences [q-bio]/Animal biology/Veterinary medicine and animal Health
Sheep
General Veterinary
Diptera
fungi
Mucin
Mucins
biology.organism_classification
medicine.disease
GS PAGE
Molecular Weight
Chromogenic Compounds
Immunoglobulin G
biology.protein
Cattle
Parasitology
Azo Compounds
Zdroj: Veterinary Parasitology
Veterinary Parasitology, Elsevier, 2003, 114 (4), pp.305-314
Veterinary Parasitology, Elsevier, 2003, 114, pp.305-314. ⟨10.1016/S0304-4017(03)00157-2⟩
ISSN: 0304-4017
Popis: The sheep bot fly, Oestrus ovis, is a very common myiasis of nasal and sinus cavities of sheep and goats causing severe welfare and production implications. As the viability of O. ovis adult flies strictly depends on larval abilities to assimilate and to stock nutrients from the host, it was necessary to investigate proteolytic activities in larval excretory/secretory products (ESP). ESP of O. ovis larvae degrade mucosal and plasmatic components such as mucin, albumin or immunoglobulin G. A preliminary biochemical characterization, using substrate gel analysis and inhibitor sensitivity, demonstrated the presence of at least six major serine proteases (molecular weights from 20 to 100 kDa), mainly trypsin-like, secreted in the digestive tube of larvae. Their involvement in larval trophic activity and evasion from the host immune response is further discussed as O. ovis excretory/secretory serine proteases could represent potential vaccinal targets.
Databáze: OpenAIRE
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