SpOT the Correct Tissue Every Time in Multi-tissue Blocks
| Autor: | Anna Coffey, Deborah L. Berry, Michael D. Johnson |
|---|---|
| Jazyk: | angličtina |
| Rok vydání: | 2015 |
| Předmět: |
Flexibility (anatomy)
Software_GENERAL General Chemical Engineering ComputingMethodologies_IMAGEPROCESSINGANDCOMPUTERVISION Biology Bioinformatics General Biochemistry Genetics and Molecular Biology Structural Biology medicine Animals Humans ComputingMethodologies_COMPUTERGRAPHICS Specimen identification Tissue microarray Paraffin Embedding General Immunology and Microbiology Orientation (computer vision) business.industry General Neuroscience Pattern recognition High throughput analysis Identification (information) Tissue sections medicine.anatomical_structure ComputingMethodologies_PATTERNRECOGNITION Tissue Array Analysis Artificial intelligence business |
| Popis: | Multi-tissue paraffin blocks provide high throughput analysis with increased efficiency, experimental uniformity, and reduced time and cost. Tissue microarrays make up the majority of multi-tissue paraffin blocks, but increasingly, researchers are using non-arrayed blocks containing larger tissues from multiple individuals which can provide many of the advantages of tissue microarrays without substantial investment in planning and equipment. A critical component of any multi-tissue analysis is the orientation method used to identify each individual tissue. Although methods exist to maintain proper orientation and identification of tissues in multi-tissue blocks, most are not well-suited to non-arrayed blocks, may consume valuable space within an array and/or are difficult to produce in the standard histology laboratory. The Specimen Orientation Tag (SpOT) is a simple, low cost orientation tool that is clearly visible in paraffin blocks and all tissue sections for reliable specimen identification in arrayed and non-arrayed layouts. The SpOT provides advantages over existing orientation methods for non-arrayed blocks as it does not require any direct modification to the tissue and allows for flexibility in the arrangement of tissue pieces. |
| Databáze: | OpenAIRE |
| Externí odkaz: |
|