High Resolution Ambient MS Imaging of Biological Samples by Desorption Electro-Flow Focussing Ionization
| Autor: | Vincen Wu, Jocelyn Tillner, Emrys Jones, James S. McKenzie, Dipa Gurung, Anna Mroz, Liam Poynter, Daniel Simon, Cristina Grau, Xavier Altafaj, Marc-Emmanuel Dumas, Ian Gilmore, Josephine Bunch, Zoltan Takats |
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| Přispěvatelé: | Medical Research Council (MRC) |
| Rok vydání: | 2022 |
| Předmět: |
Diagnostic Imaging
Spectrometry Mass Electrospray Ionization SONIC-SPRAY IONIZATION Ratolins (Animals de laboratori) Mass Spectrometry Analytical Chemistry Mice 0399 Other Chemical Sciences Electrospray ionization mass spectrometry Animals Cervell SIGNATURES Science & Technology Mass spectrometry Chemistry Analytical Brain MASS-SPECTROMETRY CANCER EXPLOSIVES Chemistry Diagnòstic per la imatge Espectrometria de masses Espectrometria de masses amb ionització per electroesprai Mice (Laboratory animals) Physical Sciences Diagnostic imaging 0301 Analytical Chemistry |
| Zdroj: | ANALYTICAL CHEMISTRY r-FSJD. Repositorio Institucional de Producción Científica de la Fundació Sant Joan de Déu instname |
| ISSN: | 1520-6882 0003-2700 |
| Popis: | In this study, we examine the suitability of desorption electro-flow focusing ionization (DEFFI) for mass spectrometry imaging (MSI) of biological tissue. We also compare the performance of desorption electrospray ionization (DESI) with and without the flow focusing setup. The main potential advantages of applying the flow focusing mechanism in DESI is its rotationally symmetric electrospray jet, higher intensity, more controllable parameters, and better portability due to the robustness of the sprayer. The parameters for DEFFI have therefore been thoroughly optimized, primarily for spatial resolution but also for intensity. Once the parameters have been optimized, DEFFI produces similar images to the existing DESI. MS images for mouse brain samples, acquired at a nominal pixel size of 50 µm, are comparable for both DESI setups, albeit the new sprayer design yields better sensitivity. Furthermore, the two methods are compared with regard to spectral intensity as well as the area of the desorbed crater on rhodamine-coated slides. Overall, the implementation of a flow focusing mechanism in DESI is shown to be highly suitable for imaging biological tissue and has potential to overcome some of the shortcomings experienced with the current geometrical design of DESI. |
| Databáze: | OpenAIRE |
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