Molecular alterations in atypical adenomatous hyperplasia occurring in benign and cancer-bearing lungs
| Autor: | Geeta S. Mantha, Sanja Dacic, Jennifer L. Hunt, Joel F. Gradowski |
|---|---|
| Rok vydání: | 2007 |
| Předmět: |
Adult
Genetic Markers Male Pathology medicine.medical_specialty Lung Neoplasms DNA Mutational Analysis Loss of Heterozygosity Adenocarcinoma medicine.disease_cause Pathology and Forensic Medicine Loss of heterozygosity Carcinoma medicine Polymorphic Microsatellite Marker Humans Atypical adenomatous hyperplasia Molecular Biology Lung Aged Aged 80 and over Mutation Adenomatosis Pulmonary business.industry Cancer Cell Biology DNA Neoplasm Middle Aged medicine.disease medicine.anatomical_structure Female business Precancerous Conditions Microsatellite Repeats |
| Zdroj: | Diagnostic molecular pathology : the American journal of surgical pathology, part B. 16(2) |
| ISSN: | 1052-9551 |
| Popis: | Atypical adenomatous hyperplasia (AAH) is considered to be a precursor lesion of the lung adenocarcinoma. Several genetic abnormalities have been reported in AAH associated with adenocarcinoma, but little is known about AAH associated with benign lung lesions. To address this we compared the molecular characteristics of AAH present in benign conditions to those coexisting with carcinoma. Seven cases of AAH from resected non-neoplastic lungs (AAH-B) and 12 cases from lungs resected for primary lung carcinoma (AAH-M) were analyzed for loss of heterozygosity (LOH) using 21 polymorphic microsatellite markers situated in proximity to known tumor suppressor genes on chromosomes 3p, 5q, 7p, 9p, 10q, and 17p. Direct DNA sequencing for K-ras mutation was also performed. There was a broad range of LOH in both groups. No LOH was identified in 3 cases (25%) of AAH-M, but all cases of AAH-B showed LOH (P=0.26). Six cases (50%) of AAH-M and 3 cases (43%) of AAH-B showed loss at 1 marker (P=0.99). LOH at 2 or more markers was identified in 3 (25%) cases of AAH-M and 4 (57%) cases of AAH-B (P=0.32). LOH was most frequently detected on chromosomes 3p and 10q in both groups. The difference in overall fractional allelic loss between the 2 groups did not reach statistical significance. K-ras mutations were not identified in either group. Our results showed a significant overlap in LOH patterns between AAH with or without coexistent lung malignancy. Therefore, AAH may represent a smoking induced low-grade neoplastic lesion that may be a precursor lesion of only a subset of invasive lung adenocarcinoma. |
| Databáze: | OpenAIRE |
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