Microfibril-associated glycoprotein-1 (MAGP-1) is specifically located on the beads of the beaded-filament structure for fibrillin-containing microfibrils as visualized by the rotary shadowing technique
| Autor: | R Polewski, Mark Gibson, Joseph C. Fanning, M Henderson |
|---|---|
| Rok vydání: | 1996 |
| Předmět: |
chemistry.chemical_classification
Histology Chemistry Microfilament Proteins Antibodies Monoclonal Fluorescent Antibody Technique Eye Fibrillins Protein filament Microscopy Electron Contractile Proteins Antibody Specificity Fibrillin Microfibrils Ultrastructure Biophysics Animals Cattle Microfibril Anatomy Glycoprotein Fibrillin |
| Zdroj: | Journal of Histochemistry & Cytochemistry. 44:1389-1397 |
| ISSN: | 1551-5044 0022-1554 |
| Popis: | This study used immunoelectron microscopic techniques to define the ultrastructural location of MAGP-1 on the fibrillin-containing microfibrils of the ocular zonule. A specific anti-MAGP-1 monoclonal antibody (MAb), 11B, was produced that did not crossreact with fibrillin-1 or other microfibrillar proteins. MAb 11B was shown by immunofluorescence to localize intensely to zonular tissue. Postembedding immunoelectron microscopy showed that MAGP-1 was associated with microfibrils throughout the zonule, with the exception of a narrow band of microfibrils at the junction with the lens capsule. With preembedding labeling, the anti-MAGP-1 MAb was found to localize in a crossbanding pattern, at intervals of about 50 nm, to microfibrils throughout the zonule and along bundles of microfibrils in surrounding vitreous tissue. Rotary shadowing of isolated microfibrils showed a "beads on a string" morphology with a periodicity of about 50 nm. With immunogold labeling, the anti-MAGP-1 antibody specifically localized on the beads in a symmetrical manner. Occasionally two gold partides were attached to the same bead, suggesting that multiple MAGP-1 molecules were present in the structure. The results indicate that MAGP-1 is intimately and regularly associated with the bead regions of fibrillin-containing microfibrils. The findings are consistent with a major structural role for MAGP-1 in microfibril biology. |
| Databáze: | OpenAIRE |
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