Inhibition of rat adrenal steroidogenesis by adenine containing compounds
| Autor: | Abdelrahman M. Moustafa, Seymour B. Koritz |
|---|---|
| Rok vydání: | 1976 |
| Předmět: |
medicine.medical_specialty
Antimycin A Endogeny Oxidative phosphorylation Biology Biochemistry Phosphates chemistry.chemical_compound Adenosine Triphosphate Endocrinology Corticosterone Internal medicine Adrenal Glands medicine Animals Atractyloside Incubation Hexokinase Succinates Ribonucleotides Rats Adenosine Diphosphate Kinetics chemistry Oligomycins Ribonucleosides Phosphoenolpyruvate carboxykinase Pyruvate kinase |
| Zdroj: | Journal of Steroid Biochemistry. 7:401-406 |
| ISSN: | 0022-4731 |
| DOI: | 10.1016/0022-4731(76)90102-3 |
| Popis: | The succinate-supported synthesis of corticosterone from endogenous precursors in a cell-free system from rat adrenals is inhibited by ADP and ATP. The inhibition is observed at short incubation times followed by a recovery from the inhibition and it appears to be specific for adenine containing compounds. The inhibition by ADP(ATP) requires phosphate, is increased by the presence of glucose plus hexokinase and eliminated by the presence of atractyloside or phosphoenolpyruvate plus pyruvate kinase. The inhibition is also eliminated if ATP is added shortly after the start of the incubation or if succinate is replaced by isocitrate. The inhibition has been found to occur only at the 11β-hydroxylation of deoxycorticosterone and the inhibition of this reaction shares many of the properties of the inhibition of corticosterone synthesis from endogenous precursors. It is concluded that the inhibition may be attributed to a competition for reducing equivalents between the hydroxylase system and oxidative phosphorylation as well as a direct effect on the hydroxylase system. |
| Databáze: | OpenAIRE |
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