Evaluation of Immunomodulatory Activities of the Heat-Killed Probiotic Strain Lactobacillus casei IMAU60214 on Macrophages In Vitro
| Autor: | Sara Centeno-Leija, Saúl Gómez-Manzo, Mariano García-Garibay, Beatriz Hernández-Ochoa, Jaime Marcial-Quino, Noemí Cárdenas-Rodríguez, Luz María Rocha-Ramírez |
|---|---|
| Rok vydání: | 2020 |
| Předmět: |
0301 basic medicine
Microbiology (medical) Lactobacillus casei medicine.medical_treatment Phagocytosis 030106 microbiology macrophage Microbiology Article law.invention 03 medical and health sciences Probiotic Immune system law Lactobacillus casei IMAU60214 Virology Lactobacillus medicine lcsh:QH301-705.5 innate immunity biology Chemistry food and beverages health lactobacillus casei imau60214 biology.organism_classification Toll-like receptors 030104 developmental biology Cytokine lcsh:Biology (General) Cytokine secretion Tumor necrosis factor alpha probiotic |
| Zdroj: | Microorganisms Volume 8 Issue 1 Microorganisms, Vol 8, Iss 1, p 79 (2020) |
| ISSN: | 2076-2607 |
| DOI: | 10.3390/microorganisms8010079 |
| Popis: | Most Lactobacillus species have beneficial immunological (&ldquo immunoprobiotic&rdquo ) effects in the host. However, it is unclear how probiotic bacteria regulate immune responses. The present study investigated the effects of heat-killed Lactobacillus casei IMAU60214 on the activity of human monocyte-derived macrophages (MDMs). Human MDMs were treated with heat-killed L. casei at a ratio (bacteria/MDM) of 50:1, 100:1, 250:1, and 500:1, and then evaluated for the following: NO production, by Griess reaction phagocytosis of FITC-labeled Staphylococcus aureus particles cytokine secretion profile (tumor necrosis factor (TNF)-&alpha interleukin (IL)-1&beta IL-6, IL-12p70, IL-10, and transforming growth factor (TGF)-&beta ) by ELISA and costimulatory molecule (CD80 and CD86) surface expression, by flow cytometry. Heat-killed L. casei IMAU60214 enhanced phagocytosis, NO production, cytokine release, and surface expression of CD80 and CD86 in a dose-dependent manner. All products were previously suppressed by pretreatment with a Toll-like receptor 2 (TLR2)-neutralizing antibody. Overall, our findings suggest that this probiotic strain promotes an M1-like pro-inflammatory phenotype through the TLR2 signaling pathway. These effects on macrophage phenotype help explain the probiotic efficacy of Lactobacillus and provide important information for the selection of therapeutic targets and treatments compatible with the immunological characteristics of this probiotic strain. |
| Databáze: | OpenAIRE |
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