Effect of long-term treatment with aromatase inhibitor on testicular function of adult male bonnet monkeys (M. radiata)
| Autor: | Hanumanthappa Krishnamurthy, H. N. Krishnamurthy, N. R. Moudgal, Gunapala Shetty, Ajay S. Bhatnagar |
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| Rok vydání: | 1998 |
| Předmět: |
Male
endocrine system medicine.medical_specialty medicine.drug_class Clinical Biochemistry Population Administration Oral Semen Biology Testicle Biochemistry DDT Time Endocrinology Internal medicine Nitriles Testis medicine Animals Testosterone Enzyme Inhibitors Spermatogenesis education Molecular Biology Pharmacology education.field_of_study Aromatase inhibitor Aromatase Inhibitors Organic Chemistry Estrogen Antagonists Molecular Reproduction Development & Genetics (formed by the merger of DBGL and CRBME) Luteinizing Hormone Triazoles Sperm Chromatin Macaca radiata medicine.anatomical_structure Estrogen Injections Intravenous Female |
| Zdroj: | Steroids. 63:414-420 |
| ISSN: | 0039-128X |
| DOI: | 10.1016/s0039-128x(98)00042-7 |
| Popis: | The role/need for estrogen in regulating testicular function of adult male bonnet monkeys (M. radiata) has been investigated by dosing orally a group of five normal males 2.5 mgs of CGP 47645, a long-acting nonsteroidal aromatase inhibitor (AI), once every 5 days for over 150 days. Such treatment resulted in a 10-fold increment in nocturnal serum testosterone (T) levels, which were sustained for 85 days of treatment, and a twofold increment in basal serum T levels was present throughout the 150 days of treatment. Analysis of ejaculated semen showed a marked reduction (approximately 90%) in sperm counts in four out of five monkeys between Days 55-85 of treatment. During this period, the motility score also was markedly reduced from a normal score of 3-5 to 0-2. Flow cytometric analysis of testicular germ cells obtained from biopsy tissue taken on Days 63 and 120 indicated a marked reduction only in elongating/elongated spermatid population (compared to Day 0 values), suggesting inhibition in spermiogenic process. Epididymal sperm maturation also seemed effected as sperm chromatin, on flow cytometric analysis for decondensability following exposure to 5 mM dithiotreitol, showed to be in a hypercondensed state. This study thus indicates that estrogen has an important role in providing normal testicular and sperm function in the primate. |
| Databáze: | OpenAIRE |
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