Overamplification of macronuclear linear DNA molecules during prolonged vegetative growth of Oxytricha nova
| Autor: | Carolyn L. Jahn, Kyungwha Song, D. S. Harper |
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| Rok vydání: | 1991 |
| Předmět: |
Time Factors
Molecular Sequence Data Oxytricha DNA Ribosomal Genome law.invention chemistry.chemical_compound law Gene duplication Genetics Animals Gene family Amino Acid Sequence Cloning Molecular Gene Ribosomal DNA Gene Library Base Sequence biology Gene Amplification Eukaryota Genetic Variation General Medicine DNA Protozoan biology.organism_classification Molecular biology chemistry RNA Ribosomal Recombinant DNA DNA |
| Zdroj: | Gene. 99:55-61 |
| ISSN: | 0378-1119 |
| Popis: | During prolonged vegetative growth of a clonal line of Oxytricha nova, several macronuclear linear DNA molecules increased greatly in copy number over the rest of the approx. 24 000 kinds of molecules comprising the macronuclear genome. One of the amplified sequences was the linear DNA molecule encoding rRNA (rDNA). We have cloned and sequenced the other, smaller, amplified molecules and found that they comprise a gene family, with different allelic versions of one of the family members being amplified. Thus, increased replication is a general property of the molecules comprising this gene family. To date, no function has been assigned to these genes; thus, whether the amplification of these sequences has functional significance is unknown. The rDNA molecule and the two small amplified sequences increased 11-, 24- and 107-fold, respectively, during clonal growth of this line, eventually comprising up to 15% of the macronuclear DNA molecules. Seven other macronuclear DNA molecules did not vary substantially in copy number at different times during the clonal growth of this strain. Analysis of cell-to-cell differences in copy numbers in this clonally aged strain indicated more extensive variation than is evident when large populations from different times are compared. |
| Databáze: | OpenAIRE |
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