Meet me halfway: Are in vitro 3D cancer models on the way to replace in vivo models for nanomedicine development?
| Autor: | Lena Neufeld, Ron Kleiner, Shani Koshrovski-Michael, Noa Reisman, Paula Ofek, Sahar Israeli Dangoor, Pradip Dey, Anna Scomparin, Ronit Satchi-Fainaro, Adva Krivitsky, Sabina Pozzi, Daniella Vaskovich-Koubi, Daniel Rodriguez Ajamil |
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| Jazyk: | angličtina |
| Předmět: |
Stromal cell
Cellular differentiation Pharmaceutical Science Antineoplastic Agents 02 engineering and technology Computational biology Biology medicine.disease_cause Metastasis 03 medical and health sciences In vivo Spheroids Cellular Neoplasms medicine Tumor Cells Cultured Animals Humans 030304 developmental biology 0303 health sciences Cultured Tissue Scaffolds business.industry Cancer 3D models Hydrogels 021001 nanoscience & nanotechnology medicine.disease hydrogel-based ECM In vitro 3. Good health Tumor Cells Nanomedicine Drug screening Immunotherapy Tumor-stromal cell interactions Personalized medicine Cellular Spheroids 0210 nano-technology business Carcinogenesis |
| Zdroj: | Advanced Drug Delivery Reviews |
| ISSN: | 0169-409X |
| DOI: | 10.1016/j.addr.2021.04.001 |
| Popis: | The complexity and diversity of the biochemical processes that occur during tumorigenesis and metastasis are frequently over-simplified in the traditional in vitro cell cultures. Two-dimensional cultures limit researchers’ experimental observations and frequently give rise to misleading and contradictory results. Therefore, in order to overcome the limitations of in vitro studies and bridge the translational gap to in vivo applications, 3D models of cancer were developed in the last decades. The three dimensions of the tumor, including its cellular and extracellular microenvironment, are recreated by combining co-cultures of cancer and stromal cells in 3D hydrogel-based growth factors-inclusive scaffolds. More complex 3D cultures, containing functional blood vasculature, can integrate in the system external stimuli (e.g. oxygen and nutrient deprivation, cytokines, growth factors) along with drugs, or other therapeutic compounds. In this scenario, cell signaling pathways, metastatic cascade steps, cell differentiation and self-renewal, tumor-microenvironment interactions, and precision and personalized medicine, are among the wide range of biological applications that can be studied. Here, we discuss a broad variety of strategies exploited by scientists to create in vitro 3D cancer models that resemble as much as possible the biology and patho-physiology of in vivo tumors and predict faithfully the treatment outcome. |
| Databáze: | OpenAIRE |
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