Mapping of the region of the tick-borne encephalitis virus replicase adjacent to initiating substrate binding center
| Autor: | Evgeny Zaychikov, A.A. Mustaev, Pletnev Ag, N.A. Belyavskaya, Yu.I. Wolf, O.V. Morozova, Kvetkova Ea |
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| Rok vydání: | 1990 |
| Předmět: |
viruses
Biophysics RNA-dependent RNA polymerase Hydroxylamine Hydroxylamines Biochemistry Peptide Mapping Virus Encephalitis Viruses Tick-Borne Adenosine Triphosphate Structural Biology Genetics Cyanogen Bromide Molecular Biology chemistry.chemical_classification NS3 Binding Sites biology Molecular mass Flavivirus RNA Affinity labelling Affinity Labels Cell Biology biology.organism_classification RNA-Dependent RNA Polymerase Virology Molecular biology Peptide Fragments Amino acid Replicase Tick-borne encephalitis virus chemistry Active center |
| Zdroj: | FEBS letters. 277(1-2) |
| ISSN: | 0014-5793 |
| Popis: | Affinity labelling with aldehyde-containing analogs of initiation substrates of nuclear fraction of tick-borne encephalitis virus (TBEV) infected cells results in a labelling of a single polypeptide with a molecular mass of 68 kDa which was immunologically identified as TBEV NS3 protein. A single-hit hydroxylamine hydrolysis, using limited and long-term CNBr cleavages allowed one to identify Lys1800 and/or Lys1803 as the label attachment sites. These amino acid residues are situated in the proximity of the ‘B’-site of NTP-binding motif of viral RNA replicase. |
| Databáze: | OpenAIRE |
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