Characterization of dextrans produced by Lactobacillus mali CUPV271 and Leuconostoc carnosum CUPV411
| Autor: | Mónica Cobos, Alicia Prieto, Patricia Ruas-Madiedo, Ana Isabel Puertas, Cristina Marieta, Paloma López, Mª Teresa Dueñas, María Goretti Llamas-Arriba, José I. Miranda |
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| Přispěvatelé: | Eusko Jaurlaritza, Ministerio de Ciencia e Innovación (España), Prieto, Alicia [0000-0002-5075-4025], López, Paloma [0000-0001-8755-8952], Marieta, Cristina [0000-0001-5222-7213], Ruas-Madiedo, Patricia [0000-0001-6158-9320], Dueñas, María Teresa [0000-0001-7551-7037], Prieto, Alicia, López, Paloma, Marieta, Cristina, Ruas-Madiedo, Patricia, Dueñas, María Teresa |
| Rok vydání: | 2019 |
| Předmět: |
General Chemical Engineering
Characterization Polysaccharide 01 natural sciences chemistry.chemical_compound 0404 agricultural biotechnology Lactobacillus 0103 physical sciences Leuconostoc Food science Dextran chemistry.chemical_classification Molar mass 010304 chemical physics biology Molecular mass Chemistry 04 agricultural and veterinary sciences General Chemistry biology.organism_classification 040401 food science Adhesion Leuconostoc carnosum Bacteria Food Science |
| Zdroj: | Digital.CSIC. Repositorio Institucional del CSIC instname |
| Popis: | 35 p.-14 fig.-4 tab. The exopolysaccharide (EPS)-producing Lactobacillus mali CUPV271 and Leuconostoc carnosum CUPV411 were isolated from Spanish ropy apple must and slimy ham, respectively. The polymers were purified from bacterial cultures’ supernatants and subjected to physicochemical and rheological characterization with the aim to evaluate their potential for future industrial utilization. Methylation analysis, Fourier-Transform Infrared Spectroscopy (FT-IR) and Nuclear Magnetic Resonance (NMR) revealed that both polymers were dextrans, partially branched at O-3 and O-4 positions of the main α-(1 → 6)-d-glucopyranose backbone. The molar masses of the EPS of L. mali and Lc. carnosum were of 1.23 × 108 g/mol and 3.58 × 108 g/mol, respectively. The bacterial strains were tested for binding to the human Caco-2 cell line in the presence and absence of their respective dextran, revealing that the EPS production by L. mali decreased the binding capacity of the bacterium while the adhesiveness of Lc. carnosum did not change. As the structure and molecular mass of both dextrans were comparable, other characteristics of the dextrans were studied to explain this behavior. Atomic force micrographs showed some differences at the supramolecular level, suggesting that the different spatial distribution of the dextrans might be on the basis of the results of the adhesion studies. Both polysaccharides resulted to be amorphous materials with Tg around 226 °C and showed slightly different thermal degradation patterns. Rheologically, they showed to have a pseudoplastic behavior, but very different critical concentrations: 3.8% for the EPS of L. mali and 0.4% for that of Lc. carnosum. This work was supported by a grant from de Department of Environment, Territorial Planification, Agriculture and Fishing from the Basque Government, by the Provincial Council of Gipuzkoa (Exp. 77/17) and by the Spanish Ministry of Science and Innovation (AGL 2015-65010-C3-1-R). |
| Databáze: | OpenAIRE |
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