Chemokine synthesis in the HSV-1-infected cornea and its suppression by interleukin-10
| Autor: | Xiao-Tian Yan, Hao Cheng, Robert N. Lausch, Terrence M. Tumpey, John E. Oakes |
|---|---|
| Rok vydání: | 1998 |
| Předmět: |
CD4-Positive T-Lymphocytes
Chemokine Time Factors Chemokine CXCL2 Immunology Herpesvirus 1 Human medicine.disease_cause Polymerase Chain Reaction Antibodies Virus Keratitis Cornea Mice medicine Animals Immunology and Allergy Macrophage Chemokine CCL4 Cells Cultured Chemokine CCL2 Chemokine CCL3 Mice Inbred BALB C biology Monokines Monocyte Immunization Passive Cell Biology Macrophage Inflammatory Proteins medicine.disease Immunohistochemistry Interleukin-10 Chemotaxis Leukocyte Interleukin 10 medicine.anatomical_structure Herpes simplex virus Keratitis Herpetic biology.protein Female Chemokines |
| Zdroj: | Journal of Leukocyte Biology. 63:486-492 |
| ISSN: | 1938-3673 0741-5400 |
| DOI: | 10.1002/jlb.63.4.486 |
| Popis: | Herpes simplex virus type 1 (HSV-1) infection of the murine cornea results in a tissue-destructive inflammatory response. In this study we show that virus infection induces the synthesis of macrophage inflammatory protein-2 (MIP-2), MIP-1α, and monocyte chemoattractant protein-1 (MCP-1). However, only the production of MIP-2 and MIP-1α coincided with the influx of leukocytes into the cornea. IL-10 treatment markedly suppressed chemokine message and protein synthesis in vivo. Local administration of IL-10 also dramatically reduced the number of T cells and neutrophils migrating into the cornea and suppressed the severity of corneal disease. The inflammatory response could also be suppressed by the passive transfer of neutralizing antibody to MIP-1α but not MCP-1. We conclude that local IL-10 administration can suppress chemokine synthesis, thereby ameliorating corneal disease. Furthermore, our results indicate that MIP-1α plays a major role in herpes stromal keratitis development, whereas MCP-1 does not. |
| Databáze: | OpenAIRE |
| Externí odkaz: |
|
Plný text