Identification and localization of a β‐COP‐like protein involved in the morphodynamics of the plant Golgi apparatus
| Autor: | Spencer Brown, Isabelle Couchy, Marie-Thérèse Crosnier, Susanne Bolte, Béatrice Satiat-Jeunemaitre |
|---|---|
| Přispěvatelé: | Institut des sciences du végétal (ISV), Centre National de la Recherche Scientifique (CNRS), La plante et son environnement (PSE), Institut National de la Recherche Agronomique (INRA)-Université Paris-Sud - Paris 11 (UP11)-Institut National Agronomique Paris-Grignon (INA P-G)-Centre National de la Recherche Scientifique (CNRS) |
| Rok vydání: | 2003 |
| Předmět: |
0106 biological sciences
Physiology Cells MESH: Plant Extracts MESH: Plant Roots Fluorescent Antibody Technique Golgi Apparatus Plant Science Biology Coatomer Protein Plant Roots 01 natural sciences MESH: Mammals MESH: Golgi Apparatus 03 medical and health sciences symbols.namesake chemistry.chemical_compound MESH: Coatomer Protein Tobacco [SDV.BV]Life Sciences [q-bio]/Vegetal Biology Animals MESH: Animals Secretion Endomembrane system MESH: Tobacco MESH: Fluorescent Antibody Technique Cells Cultured Plant Proteins 030304 developmental biology Mammals Protein Synthesis Inhibitors 0303 health sciences Golgi membrane Cultured Brefeldin A MESH: Brefeldin A MESH: Protein Synthesis Inhibitors MESH: Plant Proteins Plant Extracts Cell plate COPI Golgi apparatus Cell biology chemistry Coatomer symbols MESH: Cells Cultured 010606 plant biology & botany |
| Zdroj: | J Exp Bot J Exp Bot, 2003, 54 (390), pp.2053-63. ⟨10.1093/jxb/erg230⟩ |
| ISSN: | 1460-2431 0022-0957 |
| DOI: | 10.1093/jxb/erg230 |
| Popis: | This paper examines the molecular machinery involved in membrane exchange within the plant endomembrane system. A study has been undertaken on beta-COP-like proteins in plant cells using M3A5, an antibody raised against the conserved sequence of mammalian beta-COP proteins. In mammalian cells, beta-COP proteins are part of a complex named the coatomer, which probably recruits some specific areas of the endomembrane system. Immunofluorescence analyses by confocal laser scanning microscopy showed that beta-COP-like proteins marked predominantly the plant Golgi apparatus. Other proteins known to be part of a potential machinery for COPI vesicle formation (gamma-COP, beta'-COP and Arf1 proteins) were immunolocalized on the same membraneous structures as beta-COP. Moreover, beta-COP and other COPI antibodies stained the cell plate in dividing cells. It is further shown that, in maize root cells, and in contrast to observations upon mammalian cells, the drug Brefeldin A (BFA) does not induce the release of beta-COP and Arf1 proteins from the Golgi membrane into the cytosol. These data clearly demonstrate that the antibody M3A5 is a valuable marker for studies on trafficking events in plant cells. They also report for the first time the location of COP components in plant tissue at the light level, especially on a model well known for secretion, i.e. the maize root cells. They also suggest that the membrane recruitment machinery may function in a plant-specific way. |
| Databáze: | OpenAIRE |
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